Airflow limitation in chronic bronchitis is associated with T-lymphocyte and macrophage infiltration of the bronchial mucosa
Airflow limitation in chronic bronchitis is associated with T-lymphocyte and macrophage infiltration of the bronchial mucosa
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DOI：
10.1164/ajrccm.153.2.8564109
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发表时间：
1996-02-01
影响因子：
24.7
通讯作者：
Saetta, M
中科院分区：
文献类型：
--
作者：
DiStefano, A;Turato, G;Saetta, M
为了调查伴有气流受限的慢性支气管炎患者和不伴有气流受限的慢性支气管炎患者的气道炎症过程是否不同，我们对 14 名有慢性排痰且固定气道阻塞的受试者以及 10 名有慢性排痰和正常 FEV(1) 的受试者进行了支气管活检，所有受试者均具有吸烟史。通过免疫组织化学检查石蜡包埋和冷冻的支气管活检，以确定中性粒细胞（中性粒细胞弹性蛋白酶）、嗜酸性粒细胞（抗嗜酸性粒细胞阳离子蛋白[EG-2]）、肥大细胞（类胰蛋白酶）、T淋巴细胞（CD3）、T淋巴细胞亚群（CD4和CD8）、B淋巴细胞和巨噬细胞（CD68）的数量。粘膜下层。患有气流受限的慢性支气管炎受试者比没有气流受限的慢性支气管炎受试者具有更多的 T 淋巴细胞 (p < 0.01) 和巨噬细胞 (p < 0.05) 数量，而两组中的 T 淋巴细胞亚群以及 B 淋巴细胞、中性粒细胞、嗜酸性粒细胞和肥大细胞的数量相似。当所有受试者一起考虑时，T 淋巴细胞数量与 FEV(1) 值呈负相关（r = 0.46，p < 0.02）。总之，慢性支气管炎患者的气流受限与支气管粘膜中 T 淋巴细胞和巨噬细胞数量的增加有关。
To investigate whether the airway inflammatory process is different in patients with chronic bronchitis with airflow limitation and those with chronic bronchitis without airflow limitation, we obtained bronchial biopsies from 14 subjects with chronic sputum production and fixed airway obstruction, and from 10 subjects with chronic sputum production and normal FEV(1), all with a history of cigarette smoking. Paraffin-embedded and frozen bronchial biopsies were examined by immunohistochemistry to identify the number of neutrophils (neutrophil-elastase), eosinophils (antieosinophil cationic protein [EG-2]), mast cells (tryptase), T-lymphocytes (CD3), T-lymphocyte subpopulations (CD4 and CD8), B-lymphocytes, and macrophages (CD68) in the submucosa. Subjects with chronic bronchitis with airflow limitation had a greater number of T-lymphocytes (p < 0.01) and macrophages (p < 0.05) than subjects with chronic bronchitis without airflow limitation, whereas the T-lymphocyte subpopulations and the numbers of B-lymphocytes, neutrophils, eosinophils, and mast cells were similar in the two groups. When all the subjects were considered together, the number of T-lymphocytes correlated inversely with the values of FEV(1) (r = 0.46, p < 0.02). In conclusion, airflow limitation in subjects with chronic bronchitis is associated with an increased number of T-lymphocytes and macrophages in the bronchial mucosa.