Monoclonal antibodies identify novel neural antigens.
Monoclonal antibodies identify novel neural antigens.
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单克隆抗体可识别新的神经抗原。
DOI：
10.1073/pnas.79.7.2410
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发表时间：
1982
影响因子：
11.1
通讯作者：
A. Matus
中科院分区：
文献类型：
--
作者：
R. Hawkes;E. Niday;A. Matus
针对大鼠小脑突触质膜产生单克隆抗体 (Mab)。用固相免疫测定法筛选杂交瘤，通过小脑上的免疫过氧化物酶染色模式来表征阳性细胞系，并在蛋白质印迹上鉴定特异性多肽抗原。在所描述的 Mab 中，有些仅对神经元或神经胶质细胞进行染色，而另一些则与细胞的特定部分（例如轴突、树突和突触）发生反应。许多单克隆抗体揭示了抗原与其所在细胞之间的新关系。例如，一种名为 7D5 的单克隆抗体可与超过 30 种蛋白质的家族发生反应，但仅对神经胶质细胞进行染色。几种单克隆抗体对小脑皮质中突触大小和分布的点状位点进行染色，但每种单克隆抗体与不同的多肽子集发生反应。最受限制的细胞学染色模式之一是 12D5，它对小脑皮质颗粒层中的点状位点进行染色，并与表观 Mr 270,000 的单个多肽带发生反应。这些结果说明了培育可用于追踪大脑发育过程中特定基因产物表达的单克隆抗体的可行性。
Monoclonal antibodies (Mabs) were raised against synaptic plasma membranes from rat cerebellum. The hybridomas were screened with a solid-phase immunoassay, the positive lines were characterized by their immunoperoxidase staining pattern on cerebellum, and the specific polypeptide antigens were identified on protein blots. Among the Mabs described are some that stain only neurons or only glia and others that react with specific parts of cells, such as axons, dendrites, and synapses. Many Mabs reveal novel relationships between antigens and the cells in which they occur. For example, a Mab designated 7D5 reacts with a family of greater than 30 proteins but stains only glial cells. Several Mabs stain punctate sites of synaptic size and distribution in the cerebellar cortex but each reacts with a different subset of polypeptides. One of the most restricted cytological staining patterns is given by 12D5, which stains punctate sites in the granular layer of the cerebellar cortex and reacts with a single polypeptide band of apparent Mr 270,000. These results illustrate the feasibility of raising Mabs that can be used to follow the expression of specific gene products during brain development.