A porcine deep dermal partial thickness burn model with hypertrophic scarring
A porcine deep dermal partial thickness burn model with hypertrophic scarring
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DOI：
10.1016/j.burns.2006.02.023
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发表时间：
2006-11-01
期刊：
影响因子：
2.7
通讯作者：
Kimble, Roy M.
中科院分区：
文献类型：
--
作者：
Cuttle, Leila;Kempf, Margit;Kimble, Roy M.
我们建立了一种可复制的幼年大白猪真皮深部烧伤模型。接触灼伤是在92摄氏度的水中对15 S造成的，瓶子的底部用塑料薄膜代替。损伤深度由组织病理学家在损伤后2天和6天盲法检查组织切片。创面形成时，圆形创面形成白色焦痂，创缘周围有充血区。动物饲养6周或99天，观察创面愈合过程。伤口需要3到5周的时间才能完全再上皮化。大多数伤口形成收缩的、紫色的、肥大的疤痕。经测量，伤后6周烧伤皮肤厚度约为对照皮肤的1.8倍，伤后99天烧伤皮肤厚度约为对照皮肤的2.2倍。我们开发了各种方法来评估愈合的伤口，包括数字摄影分析，组织肉芽组织的深度，免疫组织化学，电子显微镜和张力测量。免疫组织化学和电子显微镜显示，猪增生性瘢痕与人类增生性瘢痕相似。这一模型的开发使我们能够测试和比较烧伤创面的不同治疗方法。(C)2006年爱思唯尔有限公司和ISBI。版权所有。
We developed a reproducible model of deep dermal partial thickness burn injury in juvenile Large White pigs. The contact burn is created using water at 92 degrees C for 15 s in a bottle with the bottom replaced with plastic wrap. The depth of injury was determined by a histopathologist who examined tissue sections 2 and 6 days after injury in a blinded manner. Upon creation, the circular wound area developed white eschar and a hyperaemic zone around the wound border. Animals were kept for 6 weeks or 99 days to examine the wound healing process. The wounds took between 3 and 5 weeks for complete re-epithelialisation. Most wounds developed contracted, purple, hypertrophic scars. On measurement, the thickness of the burned skin was approximately 1.8 times that of the control skin at week 6 and approximately 2.2 times thicker than control skin at 99 days after injury. We have developed various methods to assess healing wounds, including digital photographic analysis, depth of organising granulation tissue, immunohistochemistry, electron microscopy and tensiometry. Immunohistochemistry and electron microscopy showed that our porcine hypertrophic scar appears similar to human hypertrophic scarring. The development of this model allows us to test and compare different treatments on burn wounds. (c) 2006 Elsevier Ltd and ISBI. All rights reserved.