RFLP ANALYSIS OF ZEA-MAYS CALLUS-CULTURES AND THEIR REGENERATED PLANTS
RFLP ANALYSIS OF ZEA-MAYS CALLUS-CULTURES AND THEIR REGENERATED PLANTS
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DOI：
10.1007/bf00215727
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发表时间：
1991-01-01
影响因子：
5.4
通讯作者：
LORZ, H
中科院分区：
文献类型：
--
作者：
BROWN, PTH;GOBEL, E;LORZ, H
玉米自交系A188的组织培养导致与种子生长的对照植物相比具有高水平的表型变异的植物的再生。 为了确定如何诱导这种变异以及这是否与特定的体外培养方法有关，在不同的常用培养基上建立并维持愈伤组织培养物。 再生植株，并分析愈伤组织培养物和再生体的基因组DNA的RFLP差异。 结果表明，无论使用的基因探针，愈伤组织的形成导致显着偏离的DNA模式通常发现在种子生长的对照植物。 基因拷贝数也发生了变化。 随着分化和器官形成的开始，DNA变异水平下降，大多数再生植株表现出与对照的遗传相似性;那些具有RFLP差异的是体细胞克隆变异体。
Tissue culture of the Zea mays inbred line A188 resulted in the regeneration of plants having a high level of phenotypic variation compared to seed-grown control plants. To determine how such variation was induced and whether this could be related to specific in vitro culture methods, callus cultures were established and maintained on different, commonly used culture media. Plants were regenerated and the genomic DNA of callus cultures and regenerants analysed for RFLP differences. The results show that regardless of the gene probe used, callus formation resulted in significant deviations from the DNA pattern normally found in seed-grown control plants. Alterations in gene copy number also occurred. As differentiation and organogenesis began, the level of DNA variation fell, and most of the regenerated plants showed a genetic similarity to the controls; those with RFLP differences were the somaclonal variants.