Macrophage density in the pregnant rat uterine cervix is modulated by mast cell degranulation
Macrophage density in the pregnant rat uterine cervix is modulated by mast cell degranulation
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DOI：
10.1016/j.jri.2005.01.002
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发表时间：
2005-04-01
影响因子：
3.4
通讯作者：
Luque, EH
中科院分区：
文献类型：
--
作者：
Bosquiazzo, VL;Durando, M;Luque, EH
足月子宫颈经历类似炎症过程的组织形态变化。本研究的目的是更好地描述这些变化，描述巨噬细胞和肥大细胞(MC)在宫颈中分布的时间和空间模式，并评估这两种细胞是否在血管生成中发挥协同作用。用免疫组织化学方法检测周期、孕期和产后大鼠宫颈组织中巨噬细胞和巨噬细胞的表达。为抑制MC脱颗粒，给妊娠大鼠注射色甘酸钠。同时检测巨噬细胞血管内皮生长因子(VEGF)的表达。结果表明，巨噬细胞密度在分娩前升高，产后下降，MC密度则相反。有趣的是，用色甘酸二钠处理的大鼠巨噬细胞数量增加。在对照组和处理组的巨噬细胞中均未检测到血管内皮生长因子的表达；然而，不能排除MC和巨噬细胞在血管生成方面的协同作用。本研究提供了巨噬细胞和巨噬细胞在大鼠宫颈中的密度和分布的详细图谱。此外，在妊娠过程中，巨噬细胞与MC之间存在联系，并提出了MC脱颗粒调节大鼠宫颈巨噬细胞密度的证据。(C)2005爱思唯尔爱尔兰有限公司。保留所有权利。
The uterine cervix at term undergoes histomorphological changes that resemble an inflammatory process. The aim of this study was to better characterize these changes, describing the temporal and spatial pattern of macrophages and mast cells (MC) distribution in the uterine cervix and assessing whether both cells exert a coordinated action on angiogenesis. Macrophages and MC were identified by immunohistochemistry in cervical tissue from cycling, pregnant and postpartum rats. In order to inhibit MC degranulation, pregnant rats were injected with disodium cromoglycate. The expression of vascular endothelial growth factor (VEGF) by macrophages was also evaluated. Results showed that macrophage density increased towards parturition and declined at postpartum, whereas MC density showed an inverse pattern. Interestingly, disodium cromoglycate-treated rats showed an increased number of macrophages. VEGF expression in macrophages was detected neither in control nor in treated animals; however, a coordinated action between MC and macrophages on angiogenesis could not be excluded. The present study provides a detailed mapping of macrophage and MC densities and distribution in the rat uterine cervix. Moreover, an association between macrophages and MC along pregnancy is shown, and evidence that macrophage density in the rat cervix is modulated by MC degranulation is presented. (c) 2005 Elsevier Ireland Ltd. All rights reserved.