An autoradiographic analysis of neurogenesis in juvenile Aplysia californica.
An autoradiographic analysis of neurogenesis in juvenile Aplysia californica.
复制标题
加州海兔幼年神经发生的放射自显影分析。
DOI：
10.1002/neu.480220402
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Carew,TJ
中科院分区：
文献类型：
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作者：
Hickmott,PW;Carew,TJ
在发育中，新神经元的急剧增殖发生在整个中枢神经系统（CNS）中，令人惊讶的是在幼年发育的后期（Cash和Carew，1989）。在本研究中，我们调查了这些新神经元的来源。使用氚化胸苷放射自显影，我们检查了两个不同的幼年期：第11期（大规模增殖之前）和第12期（增殖高峰期）。先前的研究结果表明，在发育性失语症中，体壁是神经元的来源（麦卡利斯特，舍勒，Kandel，and阿克塞尔，1983; Jacob，1984）。因此，我们将注意力集中在邻近特定中央神经节（腹神经节）的体壁上。我们发现，在第11阶段，整个体壁的细胞标记是均匀的。然而，在第12阶段，有显着更多的标记在体壁区域紧邻腹神经节相比，侧翼区域。因此，在神经元增殖的时间，具体的和高度本地化的区域的体壁直接对他们的目标在中枢神经系统中显示出显着增加细胞division.We还研究了标记细胞的分布在腹神经节在生存时间的1和7天后胸苷注射。在阶段11和阶段12中，神经节表面上的标记细胞的分数随着时间的推移而减少，在内部观察到的分数相应地显著增加。我们的研究结果支持这样的假设，即在青少年发育中，体壁的特定区域显著上调，引起广泛的神经元增殖。然后，这些神经元从体壁迁移到它们的目标神经节，并从那里继续迁移到神经节中，以达到它们的最终位置。
In developingAplysia californica, a dramatic proliferation of new neurons occurs throughout the central nervous system (CNS) surprisingly late in juvenile development (Cash and Carew, 1989). In the present study, we investigated the source of these new neurons. Using tritiated thymidine autoradiography, we examined two different juvenile stages: stage 11 (before the large‐scale proliferation)and stage 12 (at the peak of proliferation). Previous results implicated the body wall as a source for neurons in developingAplysia(McAllister, Scheller, Kandel, and Axel, 1983; Jacob, 1984). Thus, we focused our attention on the body wall adjacent to a specific central ganglion, the abdominal ganglion. We found that in stage 11 there was uniform labelling of cells across the entire body wall. However, in stage 12 there was significantly more labelling in the body wall region immediately adjacent to the abdominal ganglion compared to flanking regions. Thus, at the time of neuronal proliferation, specific and highly localized regions of the body wall immediately opposite their target in the CNS show a significant increase in cell division.We also examined the distribution of labelled cells in the abdominal ganglion at survival times of 1 and 7 days after thymidine injection. In both stage 11 and stage 12, the fraction of labelled cells on the surface of the ganglion decreased over time, with a corresponding significant increase in the fraction observed on the inside. Our results support the hypothesis that specific regions of body wall are significantly up‐regulated in juvenileAplysiadevelopment, giving rise to widespread neuronal proliferation. These neurons then migrate from the body wall to their target ganglion, and from there continue migrating into the ganglion to achieve their final position.