Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes.
Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes.
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DOI：
10.3389/fmolb.2023.1281062
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发表时间：
2023
影响因子：
5
通讯作者：
中科院分区：
文献类型：
--
作者：
通常研究嗜热和嗜热酶对应物以了解蛋白质在恶劣条件下如何发挥作用。为了在标准温度范围之外良好地发挥作用，嗜热菌通常通过蛋白质内通讯（通过变构）和改变与配体的相互作用来严格调节其结构系综。近年来，变构串扰的增强或减弱可以是温度依赖性的，并且可以将嗜热酶与它们的嗜温旁系同源物区分开，这一点也变得很明显。由于大多数变构研究利用pH值，突变或配体的化学修饰，温度对变构功能的影响相对不足。在这里，我们讨论的生物物理方法，以及关键的案例研究，解剖温度依赖性功能的嗜温-嗜热酶对和他们的变构调节在一个温度范围内。
Mesophilic and thermophilic enzyme counterparts are often studied to understand how proteins function under harsh conditions. To function well outside of standard temperature ranges, thermophiles often tightly regulate their structural ensemble through intra-protein communication (via allostery) and altered interactions with ligands. It has also become apparent in recent years that the enhancement or diminution of allosteric crosstalk can be temperature-dependent and distinguish thermophilic enzymes from their mesophilic paralogs. Since most studies of allostery utilize chemical modifications from pH, mutations, or ligands, the impact of temperature on allosteric function is comparatively understudied. Here, we discuss the biophysical methods, as well as critical case studies, that dissect temperature-dependent function of mesophilic-thermophilic enzyme pairs and their allosteric regulation across a range of temperatures.
增强嗜热磷酸果糖激酶中的变构抑制作用。
影响因子：
2.9
作者：
McGresham, Maria S.;Reinhart, Gregory D.
通讯作者：
Reinhart, Gregory D.