Spinal cord blood flow measurement by arterial spin labeling
Spinal cord blood flow measurement by arterial spin labeling
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DOI：
10.1002/mrm.21567
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发表时间：
2008-04-01
影响因子：
3.3
通讯作者：
Kober, Frank
中科院分区：
文献类型：
--
作者：
Duhamel, Guillaume;Callot, Virginie;Kober, Frank
脊髓（SC）血流动力学的评估，特别是SC血流（SCBF）的评估，在许多SC疾病（如缺血或脊髓损伤）的病理生理学描述和理解中起着关键作用。以前在动物中使用侵入性技术（如放射自显影或标记微球）测量SCBF；然而，到目前为止还没有磁共振技术被提出。使用预饱和FAIR（血流敏感交替倒置恢复）动脉自旋标记（ASL）技术研究了MRI定量测量小鼠SCBF的可能性。SCBF测量在小鼠的颈椎水平和脑水平进行，以便使用脑血流量（CBF）值作为内部参考。SCBF图的空间分辨率为133 x 133 mu m(2)，可以测量SC不同结构（灰质、白质和脑脊液区）内的绝对区域灌注值。SC灰质（330 +/- 90ml /100g/min）和大脑（丘脑295 +/- 22ml /100g/min）的灌注值相似。这一结果与用非磁共振技术进行的SCBF/CBF测量结果一致，为无创纵向和体内动物研究开辟了新的视角。应用于人体实验也是可能的。
The assessment of spinal cord (SC) hemodynamics, and especially SC blood flow (SCBF), plays a key role in the pathophysiological description and understanding of many SC diseases such as ischemia, or spinal cord injury. SCBF has been previously measured in animals with invasive techniques such as autoradiography or labeled microspheres; no MR technique, however, has been proposed so far. The possibility of quantitatively measuring SCBF in mice using MRI was investigated using a presaturated FAIR (flow-sensitive alternating inversion recovery) arterial spin labeling (ASL) technique. SCBF measurements were performed at the cervical level of the mouse as well as on the brain so as to use cerebral blood flow (CBF) values as internal references. With a spatial resolution of 133 x 133 mu m(2) for the SCBF maps, absolute regional perfusion values could be measured within the different structures of the SC (gray matter, white matter, and cerebrospinal fluid area). Similar perfusion values were found in SC gray matter (330 +/- 90 mL/100g/min) and in brain (295 +/- 22 mL/100g/min for thalamus). This result, in agreement with SCBF/CBF measurements performed with non-MR techniques, opens new perspectives for noninvasive longitudinal and in vivo animal studies. Application to human experiments may also be possible.