Biotransformation of 2α,5α,10β,14β-Tetraacetoxy-4(20),11-taxadiene by Cell Suspension Cultures of Catharanthus roseus
Biotransformation of 2α,5α,10β,14β-Tetraacetoxy-4(20),11-taxadiene by Cell Suspension Cultures of Catharanthus roseus
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DOI：
10.1055/s-2002-36340
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发表时间：
2002-12
期刊：
影响因子：
2.7
通讯作者：
J. Dai;Ya-jun Cui;Wei-hua Zhu;Hongzhu Guo;M. Ye;Q. Hu;Dayong Zhang;Jun-hua Zheng;De-an Guo
中科院分区：
文献类型：
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作者：
J. Dai;Ya-jun Cui;Wei-hua Zhu;Hongzhu Guo;M. Ye;Q. Hu;Dayong Zhang;Jun-hua Zheng;De-an Guo
利用长春花悬浮细胞对2 α，5 α，10 β，14 β-四乙酰氧基-4（20），11-紫杉二烯（1）进行生物转化，得到4个代谢产物。根据理化数据，分别鉴定其结构为10 β-羟基-2 α，5 α，14 β-三乙酰氧基-4（20），11-紫杉二烯（2），5 α-羟基-2 α，10 β，14 β-三乙酰氧基-4（20），11-紫杉二烯（3），6 α，10 β-二羟基-2 α，5 α，14 β-三乙酰氧基-4（20），11-紫杉二烯（4），6 α，9 α，10 β-三羟基-2 α，5 α，14 β-三乙酰氧基-4（20），11-紫杉二烯（5），其中3和5为新紫杉烷类化合物。同时考察了底物添加阶段对生物转化的影响。结果表明，在细胞生长周期的线性中期（第9 ~ 12天）给药，1的生物转化率达85.3%，2的转化率达70%。另一方面，当在线性阶段早期（第6天）添加1时，4的产率达到最高水平11.8%。
Catharanthus roseus cell suspension cultures were employed for the biotransformation of 2alpha,5alpha,10beta,14beta-tetraacetoxy-4(20),11-taxadiene ( 1), and four metabolites were obtained. Based on their physical and chemical data, the structures of the four metabolites were respectively elucidated as 10beta-hydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene (2), 5alpha-hydroxy-2alpha,10beta, 14beta-triacetoxy-4(20),11-taxadiene (3), 6alpha,10beta-dihydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene (4), and 6alpha,9alpha,10beta-trihydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene (5), among which 3 and 5 were characterized as new taxoids. The effects of the stages of substrate addition on the biotransformation were also investigated. The results revealed that the biotransformation rate for 1 reached 85.3 % and the yield of 2 70 % when 1 was administered during the mid-linear phase (9 - 12 th day) of the cell growth cycle. On the other hand, the yield for 4 reached the highest level of 11.8 % when 1 was added in the early linear phase (6 th day).