Electrochemical Stimulated Pickering Emulsion for Recycling of Enzyme in Biocatalysis
Electrochemical Stimulated Pickering Emulsion for Recycling of Enzyme in Biocatalysis
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电化学刺激皮克林乳液用于生物催化中酶的回收
DOI：
10.1021/acsami.6b09920
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发表时间：
2016-11-02
影响因子：
9.5
通讯作者：
Yuan, Jinying
中科院分区：
文献类型：
--
作者：
Peng, Liao;Feng, Anchao;Yuan, Jinying
以电化学响应性微凝胶为粒子稳定剂，制备了电位刺激型Pickering乳液，并将其用于生物催化。微凝胶由环糊精官能化的8臂聚（乙二醇）（8A PEG-CD）和二茂铁修饰的对应物（8A PEG-Fc）通过CD/Fc主客体化学构建。利用Fc的氧化还原反应，外加电位可以可逆地促进微凝胶和相应Pickering乳液的形成和变形，并已用于脂肪酶催化的三乙酸甘油酯水解和（R，S）-1-苯乙醇的动力学拆分反应。乳液的潜在刺激去稳定化实现了产物的有效分离和酶的再循环。
Potential-stimulated Pickering emulsions, using electrochemical responsive microgels as particle stabilizers, are prepared and used for biocatalysis. The microgels are constructed from cyclodextrin functionalized 8-arm poly(ethylene glycol) (8A PEG-CD) and ferrocene modified counterparts (8A PEG-Fc) via CD/Fc host guest chemistry. Taking advantage of the redox reaction of Fc, the formation and deformation of the microgels and corresponding Pickering emulsions can be reversibly stimulated by external potential, and have been used for the hydrolysis of triacetin and kinetic resolution reaction of (R,S)-1-phenylethanol catalyzed by lipases. Potential stimulated destabilization of the emulsion realizes an effective separation of the products and enzyme recycling.