Interaction of the parathyroid hormone receptor with the 14-3-3 protein
Interaction of the parathyroid hormone receptor with the 14-3-3 protein
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DOI：
10.1016/s0304-4165(02)00503-2
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发表时间：
2003-03-17
影响因子：
3
通讯作者：
Zilliacus, J
中科院分区：
文献类型：
--
作者：
Tazawa, H;Takahashi, S;Zilliacus, J
甲状旁腺激素（PTH）和PTH相关蛋白（PTHrP）的受体调节钙稳态，骨重建和骨骼发育。14-3-3蛋白与信号蛋白结合，并作为分子支架和亚细胞定位的调节剂。我们发现，甲状旁腺激素受体（PTHR）与14-3-3相互作用，蛋白质共定位在细胞内。14-3-3与含有共有14-3-3结合基序的受体的C末端尾部相互作用，但也使用另外的结合位点。蛋白激酶-A处理受体，尤其是C-末端尾减少14-3-3结合。表达的C-末端尾主要定位在细胞核中，支持可能参与先前描述的PTHR核定位的推定核定位信号的功能。PTHR和14-3-3蛋白之间观察到的相互作用意味着14-3-3可能有助于调节PTHR信号传导。(C)2002 Elsevier Science B. V.保留所有权利。
The receptor for parathyroid hormone (PTH) and PTH-related protein (PTHrP) regulates calcium homeostasis, bone remodeling and skeletal development. 14-3-3 proteins bind to signaling proteins and act as molecular scaffolds and regulators of subcellular localization. We show that the parathyroid hormone receptor (PTHR) interacts with 14-3-3 and the proteins colocalize within the cell. 14-3-3 interacts with the C-terminal tail of the receptor containing a consensus 14-3-3 binding motif, but additional binding sites are also used. Protein kinase-A treatment of the receptor and especially the C-terminal tail reduces 14-3-3 binding. The expressed C-terminal tail is primarily localized in the nucleus, supporting the function of a putative nuclear localization signal that could be involved in the previously described nuclear localization of PTHR. The observed interaction between PTHR and the 14-3-3 protein implies that 14-3-3 could contribute to regulation of PTHR signaling. (C) 2002 Elsevier Science B.V. All rights reserved.