Activation of phospholipase D activity in transforming growth factor-beta-induced cell growth inhibition
Activation of phospholipase D activity in transforming growth factor-beta-induced cell growth inhibition
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DOI：
10.1038/sj.cr.7290043
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发表时间：
2000-06
期刊：
影响因子：
44.1
通讯作者：
Bing Hong Zhou;Jun Song Chen;M. Chai;Sheng Zhao;Jun Liang;He Hua Chen;Jianguo Song
中科院分区：
文献类型：
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作者：
Bing Hong Zhou;Jun Song Chen;M. Chai;Sheng Zhao;Jun Liang;He Hua Chen;Jianguo Song
细胞调节磷脂酶D（PLD）的活性，以响应许多细胞外信号。在这里，我们研究了PLD活性参与转化生长因子-β（TGF-β 1）介导的上皮细胞生长抑制。TGF-β 1抑制MDCK、Mv 1 Lu和A-549细胞的生长。在0.4%丁醇存在下，TGF-β 1诱导磷脂酰丁醇形成增加，磷脂酰丁醇是PLD催化的独特产物。TGF-β还诱导A-549和MDCK细胞中磷脂酸（PA）水平的增加。TGF-β 1可诱导A-549和MDCK细胞中[3 H]-肉豆蔻酸标记的DAG水平升高，但对Mv 1 Lu细胞无明显影响。用[3 H]-花生四烯酸预标记的细胞中没有观察到DAG的增加。结果提示PLD激活参与TGF-β 1诱导的细胞生长抑制。
Cells regulate phospholipase D (PLD) activity in response to numerous extracellular signals. Here, we investigated the involvement of PLD activity in transforming growth factor-β (TGF-b1)-mediated growth inhibition of epithelial cells. TGF-β 1 inhibits the growth of MDCK, Mv1Lu, and A-549 cells. In the presence of 0.4% butanol, TGF-β 1 induces an increase in the formation of phosphatidylbutanol, a unique product catalyzed by PLD. TGF-b also induces an increase in phosphatidic acid (PA) level in A-549 and MDCK cells. TGF-β 1 induces an increase in the levels of DAG labeled with [3 H]-myristic acid in A-549 and MDCK cells but not in Mv1Lu cells. No increase of DAG was observed in cells prelabeled with [3 H]-arachidonic acid. The data presented suggest that PLD activation is involved in the TGF-β 1-induced cell growth inhibition.