LOOPSTRUCTURES IN SYNTHETIC OLIGONUCLEOTIDES - HAIRPIN STABILITY AND STRUCTURE STUDIED AS A FUNCTION OF LOOP ELONGATION
LOOPSTRUCTURES IN SYNTHETIC OLIGONUCLEOTIDES - HAIRPIN STABILITY AND STRUCTURE STUDIED AS A FUNCTION OF LOOP ELONGATION
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DOI：
10.1007/bf02702775
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
VANBOOM, JH
中科院分区：
文献类型：
--
作者：
HAASNOOT, CAG;DEBRUIN, SH;VANBOOM, JH
本文用核磁共振、T-跳跃和紫外技术研究了同源（部分）自互补DNA片段d（ATCCTATnTAGGAT）（n = 0-7）中发夹结构的形成。结果表明，该系列中的所有化合物都可以采用发夹状构象，尽管对于n < 3，这仅在相对低的浓度下（约0.5）发生显著量。10 μ M）。对于本发明的寡核苷酸系列，发夹形成伴随着显著不同于零的表观环焓。DNA发夹的稳定性对于四个或五个残基的环长度是最大的，而早期的实验（Tinoco等人，1973）表明六至七个残基的环长度对于RNA发夹是最有利的。这可以通过考虑A-RNA和B-DNA螺旋的几何结构差异来解释。
The formation of hairpin structures in the homologous, (partly) self-complementary DNA fragments d(ATCCTATnTAGGAT), n = 0-7, was studied by means of nuclear magnetic resonance, T-jump and ultra-violet techniques. It is shown that all compounds in the series may adopt hairpin-like conformations, albeit for n < 3 this only occurs to a significant amount at relatively low concentrations (.apprx. 10 .mu.M). For the present series of oligonucleotides, hairpin formation is accompanied by an apparent loop enthalpy significantly different from zero. The stability of the DNA hairpins turns out to be at its maximum for loop lengths of four or five residues, whereas earlier experiments (Tinoco et al., 1973) indicated that loop lengths of six to seven residues are most favourable for RNA hairpins. This is explained by considering the difference in geometry of A-RNA and B-DNA helices.