A role for F-Actin in hexokinase-mediated glucose signaling1[C][W][OA]
A role for F-Actin in hexokinase-mediated glucose signaling1[C][W][OA]
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DOI：
10.1104/pp.107.108704
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发表时间：
2007-12-01
期刊：
影响因子：
7.4
通讯作者：
Moore, Brandon D.
中科院分区：
文献类型：
--
作者：
Balasubramanian, Rajagopal;Karve, Abhijit;Moore, Brandon D.
来自拟南芥（Arabidopsis thaliana）的六角激酶1（HXK 1）在葡萄糖（Glc）信号传导和Glc磷酸化中具有双重作用。然而，HXK 1在这两个过程中的功能的细胞背景还没有得到很好的理解。在这里，我们已经表明，在正常的实验检测限，AtHXK 1是本地化连续线粒体。两种线粒体孔蛋白被鉴定为能够在体内和体外结合过表达的HXK 1蛋白。我们还发现，AtHXK 1可以与它的结构同源物，F-肌动蛋白，基于他们的共免疫沉淀从转基因植物过表达HXK 1-FLAG或从瞬时表达测定，并基于他们的定位在叶细胞冷冻固定后。这种关联可能在功能上是重要的，因为在原生质体瞬时表达测定中Glc信号被F-肌动蛋白的破坏所损害。我们还表明，葡萄糖处理拟南芥幼苗迅速和可逆地破坏细网肌动蛋白丝。肌动蛋白在HXK依赖的葡萄糖信号的可能作用进行了讨论。
HEXOKINASE1 (HXK1) from Arabidopsis (Arabidopsis thaliana) has dual roles in glucose (Glc) signaling and in Glc phosphorylation. The cellular context, though, for HXK1 function in either process is not well understood. Here we have shown that within normal experimental detection limits, AtHXK1 is localized continuously to mitochondria. Two mitochondrial porin proteins were identified as capable of binding to overexpressed HXK1 protein, both in vivo and in vitro. We also found that AtHXK1 can be associated with its structural homolog, F-actin, based on their coimmunoprecipitation from transgenic plants that overexpress HXK1-FLAG or from transient expression assays, and based on their localization in leaf cells after cryofixation. This association might be functionally important because Glc signaling in protoplast transient expression assays is compromised by disruption of F-actin. We also demonstrate that Glc treatment of Arabidopsis seedlings rapidly and reversibly disrupts fine mesh actin filaments. The possible roles of actin in HXK-dependent Glc signaling are discussed.