MULTIPLE GENES ENCODING 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE IN THE GRAM-POSITIVE POLYCHLORINATED BIPHENYL-DEGRADING BACTERIUM RHODOCOCCUS-ERYTHROPOLIS TA421, ISOLATED FROM A TERMITE ECOSYSTEM
MULTIPLE GENES ENCODING 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE IN THE GRAM-POSITIVE POLYCHLORINATED BIPHENYL-DEGRADING BACTERIUM RHODOCOCCUS-ERYTHROPOLIS TA421, ISOLATED FROM A TERMITE ECOSYSTEM
复制标题
DOI：
10.1128/aem.61.2.549-555.1995
复制
发表时间：
1995-02-01
影响因子：
4.4
通讯作者：
KUDO, T
中科院分区：
文献类型：
--
作者：
MAEDA, M;CHUNG, SY;KUDO, T
红平红球菌TA 421是从白蚁生态系统中分离到的一株能降解多种多氯联苯（PCB）的菌株。该生物体的PCB分解代谢途径的遗传和生化分析显示，有四个不同的bphC基因（bphC 1，bphC 2，bphC 3和bphC 4）编码2，3-二羟基联苯双加氧酶。如通过Southern杂交所确定的，没有一个bphC基因表现出与任何其他bphC基因的同源性。bphC 1、bphC 2和bphC 4编码具有窄底物特异性并切割Meta位第一个芳环的酶，相比之下，bphC 3编码具有宽底物特异性的间Meta切割双加氧酶。Asturias等人已经表明，密切相关的生物球红球菌P6含有三种不同的bphC基因（bphC 1、bphC 2和bpHC 3），它们编码Meta裂解双加氧酶。这些数据表明，有一个不同的家庭的bphC基因编码PCB Meta裂解双加氧酶的成员的红球菌属。
Rhodococcus erythropolis TA421was isolated from a termite ecosystem and is able to degrade a wide range of polychlorinated biphenyl (PCB) congeners. Genetic and biochemical analyses of the PCB catabolic pathway of this organism revealed that there are four different bphC genes (bphC1, bphC2, bphC3, and bphC4) which encode 2,3-dihydroxybiphenyl dioxygenases. As determined by Southern hybridization, none of the bphC genes exhibits homology to any other bphC gene. bphC1, bphC2, and bphC4 encode enzymes that have narrow substrate specificities and cleave the first aromatic ring in the meta position, In contrast, bphC3 encodes a meta cleavage dioxygenase with broad substrate specificity. Asturias et al. have Shown that the closely related organism Rhodococcus globerulus P6 contains three different bphC genes (bphC1, bphC2, and bpHC3) which encode meta cleavage dioxygenases. The data suggest that there is a diverse family of bphC genes which encode PCB meta cleavage dioxygenases in members of the genus Rhodococcus.