Mediated Electrochemical Probing: A Systems-Level Tool for Redox Biology
Mediated Electrochemical Probing: A Systems-Level Tool for Redox Biology
复制标题
介导电化学探测：氧化还原生物学的系统级工具
DOI：
10.1021/acschembio.1c00267
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发表时间：
2021
影响因子：
4
通讯作者：
Payne, Gregory F.
中科院分区：
文献类型：
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作者：
Zhao, Zhiling;Ozcan, Evrim E.;VanArsdale, Eric;Li, Jinyang;Kim, Eunkyoung;Sandler, Anthony D.;Kelly, Deanna L.;Bentley, William E.;Payne, Gregory F.
生物学使用众所周知的氧化还原机制进行能量收集（例如呼吸）、生物合成和免疫防御（例如氧化爆发），现在我们知道生物学使用氧化还原进行系统级通信。目前，我们“窃听”这种氧化还原模式的能力有限，这与我们通过更熟悉的离子电模式观察和驱动生物学的能力形成鲜明对比。在本视角中，我们认为电化学与扩散介体（电子穿梭）的耦合提供了通过其电特性访问氧化还原通信方式的独特机会。我们强调之前的研究表明介导电化学探测（MEP）可以与生物学“交流”以获取信息，甚至启动特定的生物反应（即目标基因表达）。我们认为，MEP 可能会揭示一定程度的基于氧化还原的通信，而这种通信在自然界中一直未被充分认识，并且 MEP 可以为氧化还原生物学、生物电子学、临床护理和环境科学提供新的技术方法。
Biology uses well-known redox mechanisms for energy harvesting (e.g., respiration), biosynthesis, and immune defense (e.g., oxidative burst), and now we know biology uses redox for systems-level communication. Currently, we have limited abilities to “eavesdrop” on this redox modality, which can be contrasted with our abilities to observe and actuate biology through its more familiar ionic electrical modality. In this Perspective, we argue that the coupling of electrochemistry with diffusible mediators (electron shuttles) provides a unique opportunity to access the redox communication modality through its electrical features. We highlight previous studies showing that mediated electrochemical probing (MEP) can “communicate” with biology to acquire information and even to actuate specific biological responses (i.e., targeted gene expression). We suggest that MEP may reveal an extent of redox-based communication that has remained underappreciated in nature and that MEP could provide new technological approaches for redox biology, bioelectronics, clinical care, and environmental sciences.