Enhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation
Enhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation
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DOI：
10.1055/s-0037-1613586
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发表时间：
2003-04-01
影响因子：
6.7
通讯作者：
Lijnen, HR
中科院分区：
文献类型：
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作者：
Maquoi, E;Demeulemeester, D;Lijnen, HR
为了研究 Stromelysin-1 (MMP-3) 在脂肪组织发育中的潜在作用，5 周龄雄性 MMP-3 缺陷小鼠 (MMP-3(-/-)) 和野生型 (MMP-3(+/+)) 对照小鼠被保持高脂肪饮食 (HFD) 15 周。 MMP-3(-/-)小鼠比MMP-3(+/+)小鼠食欲亢进并且体重增加更多。处死时，MMP-3(-/-)小鼠的体重显着高于MMP-3(+/+)小鼠，分离的皮下(SC)和性腺(GON)脂肪沉积物的重量也是如此。在 MMP-3(-/-) 小鼠的 GON 脂肪组织中观察到显着的脂肪细胞肥大，但在 SC 脂肪组织中没有观察到。两种基因型小鼠的空腹血糖和胆固醇水平相当，而 MMP-3(-/-) 小鼠的甘油三酯水平显着较低。内皮细胞特异性凝集素染色显示，MMP-3(-/-) 小鼠的 GON 脂肪组织中血管密度明显更高，总染色面积更大。因此，在营养诱导肥胖的小鼠模型中，MMP-3可能通过影响食物摄入和/或脂肪组织相关的血管生成来损害脂肪组织发育。
To investigate a potential role of stromelysin-1 (MMP-3) in development of adipose tissue, 5 week old male MMP-3 deficient mice (MMP-3(-/-)) and wild-type (MMP-3(+/+)) controls were kept on a high fat diet (HFD) for 15 weeks. MMP-3(-/-) mice were hyperphagic and gained more weight than the MMP-3(+/+) mice. At the time of sacrifice, the body weight of the MMP-3(-/-) mice was significantly higher than that of the MMP-3(+/+) mice, as was the weight of the isolated subcutaneous (SC) and gonadal (GON) fat deposits. Significant adipocyte hypertrophy was observed in the GON but not in the SC adipose tissue of MMP-3(-/-) mice. Fasting plasma glucose and cholesterol levels were comparable in both genotypes, whereas triglyceride levels were significantly lower in MMP-3(-/-) mice. Staining with an endothelial cell specific lectin revealed a significantly higher blood vessel density and larger total stained area in the GON adipose tissues of MMP-3(-/-) mice. Thus, in a murine model of nutritionally induced obesity, MMP-3 impairs adipose tissue development, possibly by affecting food intake and/or adipose tissue-related angiogenesis.