Effect of heritable symbionts on maternally-derived embryo transcripts.
Effect of heritable symbionts on maternally-derived embryo transcripts.
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可遗传共生体对母源胚胎转录本的影响。
DOI：
10.1038/s41598-019-45371-0
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发表时间：
2019
影响因子：
4.6
通讯作者：
Erickson,JamesW
中科院分区：
文献类型：
--
作者：
Mateos,Mariana;Silva,NadishaO;Ramirez,Paulino;Higareda-Alvear,VictorM;Aramayo,Rodolfo;Erickson,JamesW
母体传播的内共生细菌在昆虫中普遍存在。在其他有影响的表型中，节肢动物的许多可遗传共生体以通过四种生殖综合征之一操纵宿主繁殖而臭名昭著，这些综合征通常在宿主的早期发育阶段发挥作用：雄性雌性化；孤雌生殖诱导;男性杀死;和细胞质不相容（CI）。在了解生殖操纵的机制和确定共生因素方面取得了重大进展，特别是雄性杀伤和细胞质不相容。尽管如此，细胞质传播细菌是否会影响卵子或早期胚胎的母体负载成分尚未得到研究。在本研究中，我们研究了在黑腹果蝇中引起不同生殖表型的遗传内共生体是否影响早期胚胎的mRNA转录组。我们使用mRNA-seq技术评估了缺乏内共生体（对照）的果蝇胚胎与含有杀死雄性的波尔氏螺原体菌株MSRO-Br、诱导ci的沃尔巴克氏杆菌菌株mel或波尔氏螺原体菌株Hyd1的果蝇胚胎的差异表达；这是一种缺乏生殖表型的菌株，自然地与果蝇有关。我们没有发现共生体对早期胚胎mRNA组成影响的一致证据，这表明生殖操纵机制不涉及母体负载转录本的改变。此外，我们利用来自螺旋体感染的黑肠果蝇胚胎的几个可用的mRNA-seq数据集，寻找与波氏螺旋体编码的核糖体失活蛋白（RIPs）活性一致的rRNA去嘌呤化信号。我们在螺旋体处理（两种菌株）中发现了微小但具有统计学意义的果蝇rna去嘌呤化信号，但在无共生体对照或沃尔巴克氏体处理中没有，这与rip的作用一致。在雄杀菌株处理下，去嘌呤信号略强。这一结果支持了最近的一项报告，即rip诱导的损伤有助于男性胚胎死亡。
Maternally-transmitted endosymbiotic bacteria are ubiquitous in insects. Among other influential phenotypes, many heritable symbionts of arthropods are notorious for manipulating host reproduction through one of four reproductive syndromes, which are generally exerted during early developmental stages of the host: male feminization; parthenogenesis induction; male killing; and cytoplasmic incompatibility (CI). Major advances have been achieved in understanding mechanisms and identifying symbiont factors involved in reproductive manipulation, particularly male killing and cytoplasmic incompatibility. Nonetheless, whether cytoplasmically-transmitted bacteria influence the maternally-loaded components of the egg or early embryo has not been examined. In the present study, we investigated whether heritable endosymbionts that cause different reproductive phenotypes inDrosophila melanogasterinfluence the mRNA transcriptome of early embryos. We used mRNA-seq to evaluate differential expression inDrosophilaembryos lacking endosymbionts (control) to those harbouring the male-killingSpiroplasma poulsoniistrain MSRO-Br, the CI-inducingWolbachiastrainwMel, orSpiroplasma poulsoniistrain Hyd1; a strain that lacks a reproductive phenotype and is naturally associated withDrosophila hydei. We found no consistent evidence of influence of symbiont on mRNA composition of early embryos, suggesting that the reproductive manipulation mechanism does not involve alteration of maternally-loaded transcripts. In addition, we capitalized on several available mRNA-seq datasets derived fromSpiroplasma-infectedDrosophila melanogasterembryos, to search for signals of depurination of rRNA, consistent with the activity of Ribosome Inactivating Proteins (RIPs) encoded bySpiroplasma poulsonii. We found small but statistically significant signals of depurination ofDrosophilarRNA in theSpiroplasmatreatments (both strains), but not in the symbiont-free control orWolbachiatreatment, consistent with the action of RIPs. The depurination signal was slightly stronger in the treatment with the male-killing strain. This result supports a recent report that RIP-induced damage contributes to male embryo death.