Sequence Determination of Peptide by the Combined Use of Fluoresceinisothiocyanate and Phenylisothiocyanate

Sequence Determination of Peptide by the Combined Use of Fluoresceinisothiocyanate and Phenylisothiocyanate
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异硫氰酸荧光素与异硫氰酸苯酯联用测定肽序列

DOI：
10.1271/bbb1961.42.1559
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
K. Tuzimura
K. Tuzimura
中科院分区：
--
文献类型：
--
作者：
K. Muramoto;H. Kawauchi;K. Tuzimura

文献摘要

被引文献

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异硫氰酸荧光素(FITC)和异硫氰酸苯酯(PITC)联用，证明了一种测定多肽氨基酸序列的荧光手动技术。这种方法允许连续分配10个残基和5.6nmol肌红蛋白，或23个残基和45nmol.
A fluorometric manual technique has been demonstrated for determining the amino acid sequence of a polypeptide, by the combined use of fluoresceinisothiocyanate (FITC) and phenylisothiocyanate (PITC). This method permits continuous assignments of 10 residues with 5.6 nmoles of myoglobin or 23 residues with 45 nmoles.