Distribution of α and β integrin subunits in the adult rat hippocampus after pilocarpine-induced neuronal cell loss, axonal reorganization and reactive astrogliosis
Distribution of α and β integrin subunits in the adult rat hippocampus after pilocarpine-induced neuronal cell loss, axonal reorganization and reactive astrogliosis
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DOI：
10.1007/s00401-003-0733-y
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发表时间：
2003-10-01
影响因子：
12.7
通讯作者：
Lie, AA
中科院分区：
文献类型：
--
作者：
Fasen, K;Elger, CE;Lie, AA
整合素是α-异二聚体，充当细胞-细胞外基质（ECM）和细胞-细胞粘附分子。在发育过程中，它们参与轴突引导、突触发生以及星形胶质细胞的成熟和迁移。在这里，我们研究了整合素亚基 α1-α5 和 β1-β5 在毛果芸香碱诱导的癫痫持续状态 (SE) 引起的成年大鼠脑中轴突萌芽、突触发生和反应性星形胶质细胞增生中的潜在作用。在软脑膜、血管内皮细胞和软脑膜表面的星形胶质细胞中观察到α1-α5、β1、β3、β4和β5的强烈海马免疫反应性。 β2 免疫反应性仅存在于血管内皮细胞中。锥体细胞和 CA3-CA1 中间神经元以及肺门神经元在其细胞体中显示出中等程度的 α5 标记。苔藓纤维对 alpha2、beta4 和 beta5 具有免疫反应性。毛果芸香碱诱导SE后，在反应性星形胶质细胞中观察到α1、α2、α4、α5、β1、β3和β4的强免疫反应性。我们的结果表明，整合素家族的成员在海马的细胞和亚细胞区室中分布不同，并经历特定的调节模式，这对于成人大脑中损伤引起的反应性变化可能很重要。
Integrins are alphabeta-heterodimers that act as cell-extracellular matrix (ECM) and cell-cell adhesion molecules. During development, they are involved in axonal guidance, synaptogenesis, and in astrocytic maturation and migration. Here, we have examined the potential role of the integrin subunits alpha1-alpha5 and beta1-beta5 in axonal sprouting, synaptogenesis and reactive astrogliosis in the adult rat brain caused by pilocarpine-induced status epilepticus (SE). Strong hippocampal immunoreactivity of alpha1-alpha5, beta1, beta3, beta4, and beta5 was observed in the pia mater, in vascular endothelia, and in astrocytes at the pial surface. beta2 immunoreactivity was found exclusively in vascular endothelia. Pyramidal cells and interneurons of CA3-CA1, as well as hilar neurons revealed moderate alpha5 labeling in their cell bodies. Mossy fibers were immunoreactive for alpha2, beta4, and beta5. After pilocarpine-induced SE, strong immunoreactivity for alpha1, alpha2, alpha4, alpha5, beta1, beta3, and beta4 was observed in reactive astrocytes. Our results show that members of the integrin family are differently distributed in cellular and subcellular compartments of the hippocampus and undergo specific patterns of regulation, which may be important for lesion-induced reactive changes in the adult brain.