CsWRKY50 mediates defense responses to Pseudoperonospora cubensis infection in Cucumis sativus
CsWRKY50 mediates defense responses to Pseudoperonospora cubensis infection in Cucumis sativus
复制标题
CsWRKY50 介导黄瓜对古巴假霜霉感染的防御反应
DOI：
10.1016/j.plantsci.2018.11.002
复制
发表时间：
2019-02-01
期刊：
影响因子：
5.2
通讯作者：
Ren, Zhonghai
中科院分区：
文献类型：
--
作者：
Luan, Qianqian;Chen, Chunhua;Ren, Zhonghai
黄瓜(Cucumis sativus L.)是一种重要的经济蔬菜作物，经常受到古巴假霜霉(P.cubensis)的侵染，导致生长受到抑制、产量降低。 WRKY 转录因子 (TF) 在植物抗病性中发挥着关键作用。然而，人们对WRKY TF在黄瓜霜霉病抗性中的作用知之甚少。在本研究中，我们报道了 CsWRKY50（黄瓜 WRKY 亚群，位于细胞核中的 TF）在黄瓜对霜霉病的防御反应中发挥着重要作用。此外，还在 CsWRKY50 启动子中鉴定了几个参与非生物胁迫反应的推定顺式作用元件。表达分析表明，CsWRKY50 可由古巴青霉感染、非生物胁迫和多种信号分子诱导。黄瓜中 CsWRKY50 的过表达增强了植物对真菌病原体 P.cubensis 的抗性。此外，由于抗氧化酶的表达水平较高，受病原体感染的35S:CsWRKY50转基因植物中积累的ROS较少。重要的是，在古巴青霉感染后，转基因植物中一些与激素相关的防御基因的转录水平也上调，包括SA和JA反应基因以及SA合成基因。总的来说，我们的结果表明，CsWRKY50 通过多种信号通路正向调节黄瓜对黄瓜的抗病性。
The cucumber (Cucumis sativus L.), an economically important vegetable crop, is often infected by Pseudoperonospora cubensis (P. cubensis), which results in inhibited growth and reduced yield. WRKY transcription factors (TFs) play critical roles in plant disease resistance. However, little is known about the function of WRKY TFs in cucumber downy mildew resistance. In this study, we reported that CsWRKY50, a cucumber WRKY subgroup lie TF localized in the nucleus, plays an important role in cucumber defense responses to downy mildew. In addition, several putative cis-acting elements involved in abiotic stress responsiveness were also identified in the CsWRKY50 promoter. Expression analysis revealed that CsWRKY50 can be induced by P. cubensis infection, abiotic stress and diverse signaling molecules. The overexpression of CsWRKY50 in cucumber enhanced the resistance of the plant to the fungal pathogen P. cubensis. In addition, less ROS accumulated in 35S:CsWRKY50 transgenic plants infected by the pathogen due to the higher expression levels of antioxidant enzymes. Importantly, after P. cubensis infection, the transcript levels of several hormone-related defense genes were also upregulated in transgenic plants, including SA- and JA-responsive genes and SA-synthesis genes. Collectively, our results indicate that CsWRKY50 positively regulates cucumber disease resistance to P. cubensis via multiple signaling pathways.