Analysis of a four generation family reveals the widespread sequence-dependent maintenance of allelic DNA methylation in somatic and germ cells.
Analysis of a four generation family reveals the widespread sequence-dependent maintenance of allelic DNA methylation in somatic and germ cells.
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对四代家族的分析揭示了体细胞和生殖细胞中等位基因 DNA 甲基化的广泛序列依赖性维持。
DOI：
10.1038/srep19260
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Cai Zhiming
中科院分区：
文献类型：
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作者：
Tang Aifa;Huang Yi;Li Zesong;Wan Shengqing;Mou Lisha;Yin Guangliang;Li Ning;Xie Jun;Xia Yudong;Li Xianxin;Luo Liya;Zhang Junwen;Chen Shen;Wu Song;Sun Jihua;Sun Xiaojuan;Jiang Zhimao;Chen Jing;Li Yingrui;Wang Jian;Wang Jun;Cai Zhiming
同源染色体的差异甲基化是导致基因组印记和X染色体失活的一种众所周知的机制，在常染色体上的非印记区域被广泛报道。为了评估人类跨代DNA甲基化模式，我们分析了一个四代家庭的体细胞和生殖细胞的DNA甲基化组。我们发现DNA甲基化的等位基因不对称性在非印记位点普遍存在，并且可能受顺式作用遗传变异体的调节。我们还观察到，绝大多数卵泡膜调节基因座的等位基因甲基化模式在同一个体的体细胞和生殖细胞之间是共享的。这些结果证明了遗传和表观遗传变异之间的相互作用，并建议在精子发生过程中广泛的DNA甲基化的序列依赖性传输的可能性。
Differential methylation of the homologous chromosomes, a well-known mechanism leading to genomic imprinting and X-chromosome inactivation, is widely reported at the non-imprinted regions on autosomes. To evaluate the transgenerational DNA methylation patterns in human, we analyzed the DNA methylomes of somatic and germ cells in a four-generation family. We found that allelic asymmetry of DNA methylation was pervasive at the non-imprinted loci and was likely regulated bycis-acting genetic variants. We also observed that the allelic methylation patterns for the vast majority of thecis-regulated loci were shared between the somatic and germ cells from the same individual. These results demonstrated the interaction between genetic and epigenetic variations and suggested the possibility of widespread sequence-dependent transmission of DNA methylation during spermatogenesis.