DOMINANT-NEGATIVE MUTANTS OF THE CDC2 KINASE UNCOUPLE CELL-DIVISION FROM ITERATIVE PLANT DEVELOPMENT
DOMINANT-NEGATIVE MUTANTS OF THE CDC2 KINASE UNCOUPLE CELL-DIVISION FROM ITERATIVE PLANT DEVELOPMENT
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DOI：
10.1002/j.1460-2075.1995.tb00064.x
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发表时间：
1995-08-15
期刊：
影响因子：
11.4
通讯作者：
FERREIRA, P
中科院分区：
文献类型：
--
作者：
HEMERLY, A;ENGLER, JD;FERREIRA, P
由于植物细胞不移动，并且被坚硬的细胞壁包围，细胞分裂速率和模式被认为是在整个发育过程中产生新结构的直接原因。为了研究细胞分裂和形态发生之间的关系，构建了转基因烟草和拟南芥植株，表达拟南芥细胞周期的关键调控因子Cdc2a激酶的显性突变。过量产生野生型Cdc2a或加速细胞周期的突变形式的植物没有表现出明显的发育改变，相反，在拟南芥中表达的预期阻止细胞周期的突变会破坏细胞分裂，而一些烟草植物则会恢复产生这种突变蛋白。这些植物组蛋白H1激酶活性降低，细胞数量明显减少，但这些细胞体积大，分化正常，形态发生、组织发生和发育时间未受影响。结果表明，在植物中，决定形状的发育控制可以独立于细胞分裂速率而起作用。
Because plant cells do not move and are surrounded by a rigid cell wall, cell division rates and patterns are believed to be directly responsible for generating new structures throughout development, To study the relationship between cell division and morphogenesis, transgenic tobacco and Arabidopsis plants were constructed expressing dominant mutations in a key regulator of the Arabidopsis cell cycle, the Cdc2a kinase, Plants constitutively overproducing the wild-type Cdc2a or the mutant form predicted to accelerate the cell cycle did not exhibit a significantly altered development, In contrast, a mutation expected to arrest the cell cycle abolished cell division when expressed in Arabidopsis, whereas some tobacco plants constitutively producing this mutant protein were recovered. These plants had a reduced histone H1 kinase activity and contained considerably fewer cells, These cells were, however, much larger and underwent normal differentiation, Morphogenesis, histogenesis and developmental timing were unaffected. The results indicate that, in plants, the developmental controls defining shape can act independently from cell division rates.