Bacteriophages against enteropathogens: rediscovery and refinement of novel antimicrobial therapeutics.
Bacteriophages against enteropathogens: rediscovery and refinement of novel antimicrobial therapeutics.
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DOI：
10.1097/qco.0000000000000772
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发表时间：
2021-10-01
影响因子：
3.9
通讯作者：
Faherty CS
中科院分区：
文献类型：
--
作者：
León Y;Faherty CS
与传统抗微生物治疗相关的细菌中抗生素耐药性和胃肠道生态失调的惊人比率导致了对开发噬菌体作为新型治疗剂的新兴趣。在这篇综述中，我们重点介绍了一些最新进展的噬菌体治疗的发展，针对重要的肠道病原体的胃肠道。噬菌体是感染细菌的病毒，或者利用细菌机器产生新的后代，或者稳定地整合到细菌染色体中以确保病毒基因组的维持。随着合成生物学的最新进展和细菌用于保护免受噬菌体侵害的CRISPR-Cas系统的发现，新的分子应用正在使我们超越噬菌体的发现并走向创新应用，包括靶向细菌毒力因子，使用温和噬菌体以及生产噬菌体蛋白作为抗微生物剂。这些技术有望靶向肠道病原体，而不会破坏胃肠道的健康微生物群。此外，纳米颗粒技术和其他修饰的使用正在帮助研究人员规避可能限制噬菌体对抗肠道病原体的功效的恶劣胃肠道条件。这个发现和发展的时代为修饰噬菌体和克服肠道病原体的全球影响提供了巨大的潜力。
Alarming rates of antibiotic resistance in bacteria and gastrointestinal dysbiosis associated with traditional antimicrobial therapy have led to renewed interests in developing bacteriophages as novel therapeutics. In this review, we highlight some of the recent advances in bacteriophage therapeutic development targeting important enteropathogens of the gastrointestinal tract. Bacteriophages are viruses that infect bacteria, either to utilize the bacterial machinery to produce new progeny or stably integrate into the bacterial chromosome to ensure maintenance of the viral genome. With recent advances in synthetic biology and the discovery of CRISPR-Cas systems used by bacteria to protect against bacteriophages, novel molecular applications are taking us beyond the discovery of bacteriophages and towards innovative applications, including the targeting of bacterial virulence factors, the use of temperate bacteriophages, and the production of bacteriophage proteins as antimicrobial agents. These technologies offer promise to target enteropathogens without disrupting the healthy microbiota of the gastrointestinal tract. Moreover, the use of nanoparticle technology and other modifications are helping researchers circumvent the harsh gastrointestinal conditions that could limit the efficacy of bacteriophages against enteric pathogens. This era of discovery and development offers significant potential to modify bacteriophages and overcome the global impact of enteropathogens.