Expression of Drosophila homologue of senescence marker protein-30 during cold acclimation
Expression of Drosophila homologue of senescence marker protein-30 during cold acclimation
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DOI：
10.1016/s0022-1910(99)00221-8
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发表时间：
2000-07-01
影响因子：
2.2
通讯作者：
Goto, SG
中科院分区：
文献类型：
--
作者：
Goto, SG
采用减法技术研究了果蝇在中等低温（15 ℃）下冷驯化过程中的基因表达。与衰老标记蛋白30（SMP30）同源的具有Ca2+结合功能的基因在15 ℃驯化后在转录水平上上调。该基因（以下称为Dca）在从卵期开始在15 ℃饲养的个体中的表达水平也高于在25 ℃饲养的个体。此外，DCA mRNA在果蝇衰老阶段增加，尽管据报道SMP30在哺乳动物衰老阶段减少。多线染色体原位杂交结果表明，Dca基因位于3R染色体88D处。该基因的5 '端侧翼区有AP-1（SMP30的转录因子）结合位点、应激反应元件和其它转录因子结合位点。DCA的功能进行了讨论有关的可能的调节细胞内Ca~（2+）浓度。(C)2000爱思唯尔科技有限公司版权所有。
Gene expression during cold acclimation at a moderately low temperature (15 degrees C) was studied in Drosophila melanogaster using a subtraction technique. A gene homologous to senescence marker protein-30 (SMP30), which has a Ca2+-binding function, was up-regulated at the transcription level after acclimation to 15 degrees C. This gene (henceforth referred to as Dca) was also expressed at a higher level in individuals reared at 15 degrees C from the egg stage than in those reared at 25 degrees C. Moreover, DCA mRNA increased at the senescent stage in Drosophila, although SMP30 is reported to decrease at senescent stages in mammals. in situ hybridization to polytene chromosomes revealed that the Dca gene was located at 88D on chromosome 3R. The 5' flanking region of this gene had AP-1 (a transcription factor of SMP30) binding sites, stress response element and some other transcription factor binding sites. The function of DCA was discussed in relation to the possible regulation of cytosolic Ca2+ concentration. (C) 2000 Elsevier Science Ltd. All rights reserved.