Femtosecond laser-induced nucleation of protein in agarose gel
Femtosecond laser-induced nucleation of protein in agarose gel
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DOI：
10.1016/j.jcrysgro.2008.09.137
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发表时间：
2009-01-15
影响因子：
1.8
通讯作者：
Mori, Yusuke
中科院分区：
文献类型：
--
作者：
Yoshikawa, Hiroshi Y.;Murai, Ryota;Mori, Yusuke
我们在琼脂糖凝胶中进行了飞秒激光诱导蛋白质成核，以研究结晶动力学。琼脂糖凝胶的使用有助于从焦点监测激光诱导结晶，因为凝胶在低过饱和度下形成高粘度溶液，从而抑制激光诱导气泡和微晶的扩散。我们成功地在微秒到几天的时间范围内从焦点监测结晶过程。此外，通过将琼脂糖凝胶系统与荧光成像相结合，我们发现激光诱导空化气泡的收缩会导致蛋白质浓度局部增加，这可能是蛋白质成核的触发因素。 (c) 2008 Elsevier B.V. 保留所有权利。
We have performed femtosecond laser-induced nucleation of protein in agarose gel to study the crystallization dynamics. The use of agarose gel facilitates the monitoring of laser-induced crystallization from the focal point, because the gel forms a highly viscous solution at low supersaturation and thereby suppresses the diffusion of laser-induced bubbles and microcrystals. We succeeded in monitoring the crystallization process from the focal point in the time range from microseconds to days. In addition, by combining an agarose gel system with fluorescence imaging, we revealed that the shrinking of laser-induced cavitation bubbles causes a local increase of protein concentration, which could be the trigger for protein nucleation. (c) 2008 Elsevier B.V. All rights reserved.