Shared antigenic epitopes and pathobiological functions of anti-p185her2/neu monoclonal antibodies
Shared antigenic epitopes and pathobiological functions of anti-p185her2/neu monoclonal antibodies
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DOI：
10.1006/exmp.1999.2266
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发表时间：
1999-09-01
影响因子：
3.6
通讯作者：
Murali, R
中科院分区：
文献类型：
--
作者：
Zhang, HT;Wang, Q;Murali, R
We have studied two anti-p185 antibodies: the monoclonal antibody 7.16.4 and rhuMAb 4D5, which were raised against the the ectodomain of rat (p185(neu)), and the human (p185(her2/neu)) homolog, respectively. Studies on the structure of these two antibodies indicate that they share structural similarity in the variable region, especially the CDR3 region, which determines the antibody-antigen interaction. Further studies by flow cytometry revealed that 7.16.4 can compete with rhuMAb4DS for binding to the cell surface p185(her2/neu), suggesting that these two antibodies share an epitope on the p185 receptor. Furthermore, 7.16.4 can also inhibit proliferation and transformation caused by p185(her2/neu). Moreover the rhuMAb 4D5 binds to the rat p185(neu). With the observation that 7.16.4 positively stains human breast cancer tissues that overexpress p185(her2/neu), 7.16.4 may be useful for the pathological diagnosis and therapy of human tumors. (C) 1999 Academic Press.
