Characterization of the duodenal bacterial microbiota in patients with pancreatic head cancer vs. healthy controls
Characterization of the duodenal bacterial microbiota in patients with pancreatic head cancer vs. healthy controls
复制标题
胰头癌患者与健康对照者十二指肠细菌微生物群的特征
DOI：
10.1016/j.pan.2018.03.005
复制
发表时间：
2018-06-01
期刊：
影响因子：
3.6
通讯作者：
Lu, Ying-Ying
中科院分区：
文献类型：
--
作者：
Mei, Qi-Xiang;Huang, Chun-Lan;Lu, Ying-Ying
An increasing number of reports have demonstrated that there is an association between the presence of pathogenic microorganisms and pancreatic cancer. However, the role of the duodenal microbiota in pancreatic carcinogenesis remains unknown. In this study, duodenal mucosal microbiota was analyzed in 14 patients with pancreatic head cancer and 14 healthy controls using 16S rRNA gene pyrosequencing methods. Plasma endotoxin activity and the concentrations of the proinflammatory cytokine IL-6 and Creactive protein (CRP) were measured in blood samples. The urea breath test was used to detect Helicobacter pylori infections. Endoscopic duodenal mucosal biopsies were evaluated by histological examinations. Statistical comparisons of inflammatory factors revealed significantly higher levels of CRP and IL-6 in the pancreatic cancer group as compared to healthy controls. Patients with pancreatic cancer also had a higher incidence of H. pylori infections and showed mucosal changes, including villous abnormalities and diffuse inflammatory cell infiltration in the lamina propria. The sequences analysis showed that based on linear discriminant analysis effect size (LEfSe) analysis at the genus level, Acinetobacter, Aquabacterium, Oceanobacillus, Rahnella, Massilia, Delftia, Deinococcus, and Sphingobium were more abundant in the duodenal mucosa of pancreatic cancer patients, whereas the duodenal microbiotas of healthy controls were enriched with Porphyromonas, Paenibacillus, Enhydrobacter, Escherichia, Shigella, and Pseudomonas. These results reveal a picture of duodenal microbiota in pancreatic head cancer patients that could be useful in future trials investigating the role of gut microbiota in pancreatic cancer. (C) 2018 IAP and EPC. Published by Elsevier B.V. All rights reserved.
