Differential expression of the retinoic acid-metabolizing enzymes CYP26A1 and CYP26B1 during murine organogenesis
Differential expression of the retinoic acid-metabolizing enzymes CYP26A1 and CYP26B1 during murine organogenesis
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DOI：
10.1016/s0925-4773(01)00572-x
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发表时间：
2002-01-01
影响因子：
2.6
通讯作者：
Dollé, P
中科院分区：
文献类型：
--
作者：
Abu-Abed, S;MacLean, G;Dollé, P
我们最近克隆了一种新的维甲酸（RA）代谢酶Cyp26B1的小鼠同源物，并发现其在胚胎早期的表达模式与Cvp26A1不同。在这里，我们完成了从胚胎日(E) 12到出生后阶段的比较表达分析。通过E14.5， Cyp26B1在发育中的肌腱和不稳定元件以及软骨膜中表达，而Cyp26A1的表达仅限于肋骨和椎软骨的四肢。Cyp26A1和Cyp26B1分别表达于四肢远端上皮和生殖器结节间质。高Cyp26B1表达在颅面区域进行形态发生生长，而Cyp26A1信息仅限于口腔和牙齿上皮。cyp26a1在发育中的神经视网膜中单独表达，而这两个基因在视网膜色素上皮中共表达。Cyp26B1在发育中的后脑（脑桥、小脑）和前脑（纹状体、海马）中特异性表达，出生后在前脑持续表达。此外，Cyp26B1在分化的上、下胸椎脊髓中有特定水平的表达，邻近表达ra合成酶RALDH-2的颈椎和腰椎区域。在脏器中，在发育中的肺、肾、脾、胸腺和睾丸中检测到Cyp26B1转录本，而在膈肌和外胃间质中检测到Cyp26A1转录本。Cyp26B1也在发育中的毛囊周围的真皮中特异性表达。因此，许多发育中的系统可能需要调节R-A代谢。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
We recently cloned the murine homologue of Cyp26B1, a novel retinoic acid (RA)-metabolizing enzyme and showed that its a ne expression pattern is unique from that of Cvp26A1 during early embryogenesis. Here, we complete this comparative expression analysis from embryonic day (E) 12 to postnatal stages. Cyp26B1 expression was found in developing tendons and precartilaginous elements and in perichondrium by E14.5, while Cyp26A1 expression was restricted to extremities of rib and vertebral cartilage. Cyp26A1 and Cyp26B1 were expressed, in the distal epithelium and mesenchyme of the limbs and genital tubercle, respectively. High Cyp26B1 expression was found in craniofacial areas undergoing morphogenetic growth, whereas Cyp26A1 message was restricted to the mouth and dental epithelium. Cyp26A1alone was expressed in the developing neural retina, while both genes were co-expressed in the retinal pigment epithelium. Cyp26B1 was specifically expressed in the developing hindbrain (pons, cerebellum) and forebrain (striatum, hippocampus), with forebrain expression persisting postnatally. In addition, Cyp26B1 was expressed at specific levels of the differentiating upper and lower thoracic spinal cord, adjacent to the cervical and lumbar regions that express the RA-synthesizing enzyme RALDH-2. In viscera, Cyp26B1 transcripts were detected in the developing lung, kidney, spleen, thymus and testis, whereas Cyp26A1 transcripts were found in the diaphragm and outer stomach mesenchyme. Cyp26B1 was also specifically expressed in dermis surrounding the developing hair follicles. Regulated R-A metabolism may therefore be required in many developing systems. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.