Whole cell ELISA for detection of tumor antigen expression in tumor samples
Whole cell ELISA for detection of tumor antigen expression in tumor samples
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DOI：
10.1016/s0022-1759(01)00465-3
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发表时间：
2001-12-01
影响因子：
2.2
通讯作者：
Berd, D
中科院分区：
文献类型：
--
作者：
Erdile, LF;Smith, D;Berd, D
我们开发了一种全细胞酶联免疫吸附试验（ELISA），用于检测转移性黑色素瘤患者肿瘤样本中的黑色素瘤抗原gp 100和S-100。卵巢癌患者肿瘤标本中CA - 125的检测。该分析相对简单，无需专门的病理学专业知识即可执行和解释，它既敏感又有选择性，并且易于自动化。结果与流式细胞术的结果非常吻合，与已发表的免疫组织化学结果也很一致。我们相信，这种检测方法对鉴定自体细胞癌疫苗非常有帮助，也可能是癌症诊断中免疫组织化学的一种有用的替代方法。它应该适用于其他类型的癌症，肿瘤抗原已经确定，良好的抗体是可用的。(C) 2001 Elsevier Science B.V.版权所有
We have developed a whole cell enzyme-linked immunosorbent assay (ELISA) that detects the melanoma antigens gp 100 and S-100 in tumor samples from patients with metastatic melanoma and the antigen. CA 125 in tumor samples from patients with ovarian carcinoma. The assay is relatively simple to perform and interpret without specialized expertise in pathology, It is both sensitive and selective and is amenable to being automated. The results correlate very well with those obtained by flow cytometry and are in good accord with published values obtained by immunohistochemistry. We believe that this assay should be very helpful for characterizing autologous cell cancer vaccines and may also represent a useful alternative to immunohistochemistry for cancer diagnosis. It should be adaptable to other types of cancer where tumor antigens have been identified and good antibodies are available. (C) 2001 Elsevier Science B.V. All rights reserved.