Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells.
Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells.
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DOI：
10.3390/microorganisms4040040
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发表时间：
2016-10-27
期刊：
影响因子：
4.5
通讯作者：
Kaminogawa S
中科院分区：
文献类型：
--
作者：
Harata G;He F;Takahashi K;Hosono A;Miyazawa K;Yoda K;Hiramatsu M;Kaminogawa S
肥大细胞在免疫球蛋白E（IgE）介导的过敏性疾病中起关键作用，并且肥大细胞的脱粒在这些疾病的发病机制中是重要的。肠道菌群失调，特别是内源性乳酸菌的失调，可能是IgE介导的过敏性疾病的一个促成因素。关于人肠道乳酸杆菌与肥大细胞相互作用的额外知识仍然是必要的。基于β-氨基己糖苷酶释放试验，检测了23株乳杆菌（包括商业菌株和参考菌株以及来自人肠道的菌株）在体外调节大鼠嗜碱性白血病RBL-2 H3细胞（RBL-2 H3）的细胞脱粒的能力。每一种测试的乳酸杆菌特征性地抑制IgE介导的RBL-2 H3细胞的脱粒，并且乳酸杆菌GG显示出对细胞的最强抑制作用。此外，与参考菌株相比，从人肠道分离的细菌显著抑制RBL-2 H3细胞的脱粒。这些结果表明，乳酸杆菌，特别是那些从人类肠道，可以影响肥大细胞的激活，在应变依赖性的方式。理解机制有待进一步研究。
Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated allergic diseases. Additional knowledge regarding the interaction of human intestinal Lactobacilli with mast cells is still necessary. Twenty-three strains of Lactobacilli, including commercial and reference strains and strains from the human intestine, were tested for their ability to regulate degranulation of cells from rat basophilic leukemia RBL-2H3 cells (RBL-2H3) in vitro based on a β-hexosaminidase release assay. Each of the tested Lactobacilli characteristically suppressed IgE-mediated degranulation of RBL-2H3 cells, and Lactobacillus GG showed the strongest inhibitory effect on the cells. Furthermore, the bacteria isolated from the human intestine significantly suppressed degranulation of RBL-2H3 cellsin comparison with the reference strains. These results suggest that Lactobacilli, particularly those from the human intestine, can affect the activation of mast cells in a strain-dependent manner. Further study should be conducted to analyse the understanding mechanism.