Intravenous Vitamin C attenuates hemorrhagic shock-related renal injury through the induction of SIRT1 in rats
Intravenous Vitamin C attenuates hemorrhagic shock-related renal injury through the induction of SIRT1 in rats
复制标题
静脉注射维生素 C 通过诱导 SIRT1 减轻大鼠失血性休克相关的肾损伤
DOI：
10.1016/j.bbrc.2018.04.111
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发表时间：
2018-06-22
影响因子：
3.1
通讯作者：
Mao, En-Qiang
中科院分区：
文献类型：
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作者：
Qi, Meng-Zhi;Yao, Yi;Mao, En-Qiang
探讨静脉注射维生素C（VC）对失血性休克（HS）相关大鼠肾损伤的影响及其机制。将30只SD大鼠随机分为假手术(sham)组、失血性休克(HS)组、HS+100 mg/kg VC(H+VL)组、HS+500 mg/kg VC(H+VH)组和HS+100 mg/kg VC+EX527(H+VL+E)组。手术后6小时收集组织和血液样本。对肾脏病理变化进行评分。检测血清中肌酐（CRE）、血尿素氮（BUN）、肿瘤坏死因子-a（TNF-α）和白细胞介素-1β（IL-1β）水平以及血浆中维生素C水平和超氧化物歧化酶（SOD）活性以及抑制羟自由基（RAFHR）的能力。通过 ELISA 或蛋白质印迹分析组织中 Sirtuinl (SIRT1)、乙酰-NF-κ B (Ace-NF-κ B)、血红素加氧酶-1 (HO-1)、TNF-α 和 IL-1 β 的表达。 HS组的肾脏病理评分及血清中CRE、BUN、TNF-α、IL-1β的含量均显着高于Sham组（P
To investigate the effect of intravenous Vitamin C (VC) on hemorrhagic shock (HS)-associated rat renal injury and the involved mechanism. Thirty SD rats were randomly assigned to the sham surgery (sham), hemorrhagic shock (HS), HS+100 mg/kg VC (H + VL), HS+500 mg/kg VC (H + VH) and HS+100 mg/kg VC + EX527 (H + VL + E) groups. Tissue and blood samples were collected 6 h after surgery. Kidney pathological changes were scored. Creatinine (CRE), blood urea nitrogen (BUN), tumor necrosis factor-a (TNF-alpha), and interleukin-1 beta (IL-1 beta) levels in serum and Vitamin C levels and superoxide dismutase (SOD) activity and the ability to suppress hydroxyl radical (RAFHR) in plasma were measured. The expression of Sirtuinl (SIRT1), Acetyl-NF-kappa B (Ace-NF-kappa B), heme oxygenase-1 (HO-1), TNF-alpha, and IL-1 beta in tissues was analyzed by ELISA or western-blot. In the HS group, the kidney pathological score and CRE, BUN, TNF-alpha, and IL-1 beta levels in serum were significantly higher than in the Sham group (P