Polyamines are essential in embryo implantation: expression and function of polyamine-related genes in mouse uterus during peri-implantation period.
Polyamines are essential in embryo implantation: expression and function of polyamine-related genes in mouse uterus during peri-implantation period.
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DOI：
10.1210/en.2007-1420
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发表时间：
2008-05
期刊：
影响因子：
4.8
通讯作者：
Yue-chao Zhao;Y. Chi;Yong-sheng Yu;Ji-Long Liu;Ren-Wei Su;Xinghong Ma;Chun-Hua Shan;Zeng-Ming Yang
中科院分区：
文献类型：
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作者：
Yue-chao Zhao;Y. Chi;Yong-sheng Yu;Ji-Long Liu;Ren-Wei Su;Xinghong Ma;Chun-Hua Shan;Zeng-Ming Yang
多胺是细胞生长和分化的关键调节因子。研究表明，鸟氨酸脱羧酶(ODC)是着床后胚胎发育所必需的，亚精胺/精胺N1-乙酰转移酶的过度表达将导致卵巢功能减退和子宫发育不全。然而，多胺相关基因在妊娠早期小鼠子宫中的表达和功能尚不清楚。在这项研究中，我们研究了多胺相关基因在小鼠着床期子宫中的表达、调控和功能。ODC在种植部位有较强的表达，并被雌激素刺激。ODC抗酶1和精胺/精胺N1-乙酰转移酶在着床部位也有较高的表达，并受子宫细胞中ODC或多胺水平的调节。ODC抑制剂α-二氟甲基鸟氨酸显著抑制胚胎着床。此外，S腺苷甲硫氨酸脱羧酶基因的表达上调弥补了α-二氟甲基鸟氨酸处理引起的ODC活性的降低。综上所述，我们的结果表明子宫多胺相关基因的协调表达可能对胚胎着床很重要。
Polyamines are key regulators in cell growth and differentiation. It has been shown that ornithine decarboxylase (Odc) was essential for post-implantation embryo development, and overexpression of spermidine/spermine N1-acetyltransferase will lead to ovarian hypofunction and hypoplastic uteri. However, the expression and function of polyamine-related genes in mouse uterus during early pregnancy are still unknown. In this study we investigated the expression, regulation, and function of polyamine-related genes in mouse uterus during the peri-implantation period. Odc expression was strongly detected at implantation sites and stimulated by estrogen treatment. The expression of Odc antizyme 1 and spermidine/spermine N1-acetyltransferase was also highly shown at implantation sites and regulated by Odc or polyamine level in uterine cells. Embryo implantation was significantly inhibited by alpha-difluoromethylornithine, an Odc inhibitor. Moreover, the reduction of Odc activity caused by alpha-difluoromethylornithine treatment was compensated by the up-regulation of S-adenosylmethionine decarboxylase gene expression. Collectively, our results indicated that the coordinated expression of uterine polyamine-related genes may be important for embryo implantation.