Developmental function and state transitions of a gene expression oscillator inCaenorhabditis elegans
Developmental function and state transitions of a gene expression oscillator inCaenorhabditis elegans
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DOI：
10.15252/msb.20209498
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发表时间：
2020-07-01
影响因子：
9.9
通讯作者：
Grosshans, Helge
中科院分区：
文献类型：
--
作者：
Meeuse, Milou W. M.;Hauser, Yannick P.;Grosshans, Helge
基因表达振荡器可以在时间和空间上构建生物事件。不同的生物功能受益于不同的振荡器特性。因此，有限的发育过程依赖于在特定时间启动和停止的振荡器，这是一种鲜为人知的行为。在这里，我们已经表征了一个巨大的基因表达振荡器，包括>；3,700个基因在秀丽线虫幼虫。我们报告说，振荡在胚胎中开始，孵化后瞬间停止，并对扰动做出反应，然后在成体中停止。在高时间分辨率下对振荡态和非振荡态之间的跃迁的实验观测揭示了一个振子工作在不变周期(SNIC)分叉上的鞍结点附近。这些发现限制了能够代表这种振荡器的体系结构和数学模型。它们还揭示了振荡器抑制在特定的阶段重复发生。由于我们发现振荡与包括蜕皮在内的发育过程相耦合，SNIC分叉的这一特征赋予振荡器以特定的时间间隔停止幼虫发育的潜力，从而执行发育检查点功能。
Gene expression oscillators can structure biological events temporally and spatially. Different biological functions benefit from distinct oscillator properties. Thus, finite developmental processes rely on oscillators that start and stop at specific times, a poorly understood behavior. Here, we have characterized a massive gene expression oscillator comprising > 3,700 genes inCaenorhabditis eleganslarvae. We report that oscillations initiate in embryos, arrest transiently after hatching and in response to perturbation, and cease in adults. Experimental observation of the transitions between oscillatory and non-oscillatory states at high temporal resolution reveals an oscillator operating near a Saddle Node on Invariant Cycle (SNIC) bifurcation. These findings constrain the architecture and mathematical models that can represent this oscillator. They also reveal that oscillator arrests occur reproducibly in a specific phase. Since we find oscillations to be coupled to developmental processes, including molting, this characteristic ofSNICbifurcations endows the oscillator with the potential to halt larval development at defined intervals, and thereby execute a developmental checkpoint function.