Advanced glycation end products in idiopathic frozen shoulders.
Advanced glycation end products in idiopathic frozen shoulders.
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DOI：
10.1016/j.jse.2015.10.015
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发表时间：
2016-06
影响因子：
3
通讯作者：
Walton JR
中科院分区：
文献类型：
--
作者：
Hwang KR;Murrell GA;Millar NL;Bonar F;Lam P;Walton JR
特发性冻结肩中成纤维细胞增殖和致密胶原基质沉积的病理生理机制尚不清楚。晚期糖基化终末产物（AGEs）的积累与胶原的交联和稳定性已被假设为促成这一病理生理过程。本研究旨在探讨AGEs的免疫反应性是否在特发性冻结肩患者高于对照组。从8名特发性冻结肩患者、6名不稳定肩患者（对照1）和8名肩袖撕裂患者（对照2）中采集肩关节囊样本。样本进行苏木精和伊红染色，并使用抗AGEs抗体进行免疫组织化学分析。免疫反应以盲法从无（0）到强（3）进行评级。观察到囊内的免疫组织化学分布。与肩袖撕裂患者相比，冻结肩患者自我报告疼痛的频率和严重程度更高（P = 0.02），与不稳定和肩袖撕裂组患者相比，所有平面的活动范围更受限（P <0.05）。苏木素和伊红染色的冻结肩关节囊组织显示成纤维细胞增殖，脂肪细胞数量增加，滑膜下血管增多。AGEs的免疫反应性在冻结肩关节囊（2.8）中强于不稳定（0.3; P = 0.0001）和肩袖撕裂（1.1; P = 0.016）。这项研究强调了AGEs在冻结肩发病机制中的潜在作用。AGEs的过度表达可以解释特发性冻结肩成纤维细胞的增生和胶原基质的沉积。基础科学研究;组织学
The pathophysiologic mechanisms behind proliferation of fibroblasts and deposition of dense collagen matrix in idiopathic frozen shoulder remain unclear. Accumulation of advanced glycation end products (AGEs) with cross-linking and stabilization of collagen has been hypothesized to contribute to this pathophysiologic process. This study investigated whether the immunoreactivity of AGEs is higher in patients with idiopathic frozen shoulder than in the control groups. Shoulder capsule samples were collected from 8 patients with idiopathic frozen shoulder, 6 with unstable shoulders (control 1), and 8 with rotator cuff tears (control 2). The samples were hematoxylin and eosin stained and analyzed by immunohistochemistry using antibodies against AGEs. Immunoreactivities were rated in a blinded fashion from none (0) to strong (3). Immunohistochemical distribution within the capsule was noted. Frozen shoulder patients had greater frequency and severity of self-reported pain (P = .02) than rotator cuff tear patients and more restricted range of motion in all planes (P < .05) than patients of the instability and rotator cuff tear groups. Hematoxylin and eosin–stained capsular tissue from frozen shoulder showed fibroblastic proliferation, increased numbers of adipocytes, and increased subsynovial vascularity. Immunoreactivity of AGEs was stronger in frozen shoulder capsules (2.8) than in instability (0.3; P = .0001) and rotator cuff tear (1.1; P = .016) capsules. This study highlights a potential role for AGEs in the pathogenesis of frozen shoulder. The overexpression of AGEs may explain the fibroblastic proliferation and deposition of collagen matrix in idiopathic frozen shoulder. Basic Science Study; Histology