Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant.
Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant.
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DOI：
10.3390/plants4010085
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发表时间：
2015-01-21
期刊：
影响因子：
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通讯作者：
Kieliszewski MJ
中科院分区：
文献类型：
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作者：
Chen Y;Ye D;Held MA;Cannon MC;Ray T;Saha P;Frye AN;Mort AJ;Kieliszewski MJ
伸展蛋白是细胞壁富含羟脯氨酸的糖蛋白（HRGP）超家族的成员，在初生细胞壁中形成共价交联的网络。拟南芥兰茨贝格直立中编码EXTENSIN 3（EXT 3）的基因EXT 3（AT 1G 21310）的敲除突变导致致死表型，尽管约20%的敲除植物具有表观正常表型（ANP）。在这项研究中，根细胞壁HRGP组件的野生型，ANP和ext 3突变体幼苗的特点是通过胰蛋白酶消化的无水氟化氢去糖基化壁残基的肽分馏和使用LC-MS/MS测序。几个HRGP，包括EXT 3，被确定在野生型根壁，但不是在ANP和致死突变体的墙壁。事实上，显示致死表型的ANP壁和突变体的壁具有HRGP，但这些特征表明，数量和类型的变化可能是相应表型的原因。
Extensins are members of the cell wall hydroxyproline-rich glycoprotein (HRGP) superfamily that form covalently cross-linked networks in primary cell walls. A knockout mutation in EXT3 (AT1G21310), the gene coding EXTENSIN 3 (EXT3) in Arabidopsis Landsberg erecta resulted in a lethal phenotype, although about 20% of the knockout plants have an apparently normal phenotype (ANP). In this study the root cell wall HRGP components of wild-type, ANP and the ext3 mutant seedlings were characterized by peptide fractionation of trypsin digested anhydrous hydrogen fluoride deglycosylated wall residues and by sequencing using LC-MS/MS. Several HRGPs, including EXT3, were identified in the wild-type root walls but not in walls of the ANP and lethal mutant. Indeed the ANP walls and walls of mutants displaying the lethal phenotype possessed HRGPs, but the profiles suggest that changes in the amount and perhaps type may account for the corresponding phenotypes.