HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION AND PHOTOMETRIC DETECTION OF PHOSPHOLIPIDS
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION AND PHOTOMETRIC DETECTION OF PHOSPHOLIPIDS
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DOI：
10.1016/s0021-9673(01)92081-3
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
GEURTSVANKESSEL, WSM
中科院分区：
文献类型：
--
作者：
HAX, WMA;GEURTSVANKESSEL, WSM
本文介绍了一种以正己烷-异丙醇-水为溶剂的高效液相色谱法分离磷脂的快速有效方法。在硅胶柱上进行脂质分离，并通过206 nm处的UV吸收直接监测单个组分。总脂质提取物从红细胞以及suboesophageal神经节的snailHellypomatia，实现完全分离的胆固醇，磷脂酸，磷脂酰乙醇胺，磷脂酰肌醇，磷脂酰丝氨酸，溶血磷脂酰胆碱和溶血磷脂酰乙醇胺，而磷脂酰胆碱和鞘磷脂在这些情况下部分分离。除了分离不同类别的磷脂外，在某些情况下还可以实现分子种类的分离，如磷脂酰胆碱和鞘磷脂所示。
A rapid and efficient method for the separation of (phospho)lipids by high-performance liquid chromatography usingn-hexane-2-propanol-water mixtures as the solvent system is described. The lipid separation occurs on silica gel columns and the individual components are monitored directly by UV absorption at 206 nm. Of a total lipid extract from erythrocytes as well as suboesophageal ganglia of the snailHelix pomatia, a complete separation is achieved of cholesterol, phosphatidic acid, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, lysophosphatidylcholine and lysophosphatidylethanolamine, whereas phosphatidylcholine and sphingomyelin are partly separated under these circumstances. In addition to separation of phospholipids in different classes, separation of molecular species can also be achieved in some instances, as is shown for phosphatidylcholines and sphingomyelins.