No direct mitogenic effect of sex hormones on antlerogenic cells detected in vitro
No direct mitogenic effect of sex hormones on antlerogenic cells detected in vitro
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DOI：
10.1006/gcen.2001.7681
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发表时间：
2001-10-01
影响因子：
2.7
通讯作者：
Suttie, JM
中科院分区：
文献类型：
--
作者：
Li, C;Wang, W;Suttie, JM
鹿蒂是鹿角的前身，由覆盖在鹿额骨外侧嵴上的特殊骨膜（鹿角形成骨膜）发育而来。花蒂生长的开始是由雄激素触发的。到目前为止，尚不清楚蒂起始是由雄激素对致角骨膜的直接刺激引起的，还是需要一些中间机制。本研究采用体外方法研究性激素是否对原代培养的成角骨膜细胞有直接的有丝分裂作用。从两只5个月大的马鹿幼鹿身上获得了致角细胞。细胞传代两次，然后用睾酮、二氢睾酮和雌二醇处理。增殖实验显示，性激素对二代致角细胞无直接的有丝分裂作用（P < 0.05）。睾酮结合研究表明，在第二次传代中，特定的睾酮结合位点存在于致角细胞中。因此，我们得出结论，雄激素在体外对致角细胞没有有丝分裂作用。我们的研究结果表明，弓根的形成可能不是雄激素直接刺激致角组织的结果。相反，雄激素可能只通过增加致角细胞对有丝分裂原（例如某些生长因子）的敏感性来允许这一过程进行。(C) 2001学术出版社。
Deer pedicles, antecedents of antlers, develop from a specialized periosteum (antlerogenic periosteum) which overlies the lateral crest of the deer frontal bone. The initiation of pedicle growth is triggered by androgen hormones. Thus far, it is not known whether pedicle initiation is caused by direct stimulation of androgen hormones on the antlerogenic periosteum or whether some intermediate mechanisms are necessary. The present study took an in vitro approach to investigate whether sex hormones have direct mitogenic effects on primary cultured antlerogenic periosteal cells (antlerogenic cells). Antlerogenic cells were obtained from two 5-month-old red deer calves. The cells were passaged twice and then treated with testosterone, dihydrotestosterone, and estradiol. The proliferation assays showed that no direct mitogenic effects on the second passage antlerogenic cells could be detected with any of the sex hormone treatments (P > 0.05). Testosterone-binding studies showed that at the second passage, specific testosterone-binding sites were present in the antlerogenic cells. Therefore, we conclude that androgens do not have mitogenic effects on antlerogenic cells in vitro. Our results suggest that pedicle formation may not be the result of direct stimulation of androgen hormones on antlerogenic tissue. Instead, androgen hormones may only allow the process to proceed by increasing the sensitivity of antlerogenic cells to mitogens, e.g., some growth factors. (C) 2001 Acidemic Press.