Tumour-infiltrating lymphocytes and expression of programmed death ligand 1 (PD-L1) in melanoma brain metastases
Tumour-infiltrating lymphocytes and expression of programmed death ligand 1 (PD-L1) in melanoma brain metastases
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DOI：
10.1111/his.12537
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发表时间：
2015-01-01
期刊：
影响因子：
6.4
通讯作者：
Preusser, Matthias
中科院分区：
文献类型：
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作者：
Berghoff, Anna Sophie;Ricken, Gerda;Preusser, Matthias
目的在本研究中，我们旨在表征一系列黑色素瘤 BM 中免疫浸润和程序性死亡 1 (PD-1) 和程序性死亡配体 1 (PD-L1) 的表达，为使用免疫检查点抑制剂的实验治疗提供基础。方法和结果我们研究了 PD-1、PD-L1、CD3、CD8、CD45RO、叉头盒蛋白 3 (FoxP3)、CD20 和 BRAF V600E 的表达通过黑色素瘤 BM 样本中的免疫组织化学分析。可获得 43 份样本 [其中 27 份 (62.8%) 为 BRAF V600E 阳性]。 CD3(+) 肿瘤浸润淋巴细胞 (TIL) 在 33 例 (76.7%) 中明显，CD8(+) 39 例 (90.7%)，CD45RO(+) 32 例 (74.4%)，PD-1(+) 27 例 (62.8%)，FoxP3(+) 21 例 (48.8%)，CD20(+) TIL 19 (44.2%)。在 22 个样本（51.1%）中观察到肿瘤 PD-L1 表达，其中 9 个样本（40.9%）在超过 5% 的肿瘤细胞中观察到表达。 PD-L1 表达与 PD-1(+) (P< 0.001)、CD3(+) (P=0.014) 和 FoxP3(+) (P
AimsIn this study we aimed to characterize immune infiltrates and expression of programmed death 1 (PD-1) and programmed death ligand 1 (PD-L1) in a series of melanoma BM to provide a basis for experimental therapy using immune checkpoint inhibitors.Methods and resultsWe investigated expression of PD-1, PD-L1, CD3, CD8, CD45RO, forkhead box protein 3 (FoxP3), CD20 and BRAF V600E by immunohistochemistry in melanoma BM samples. Forty-three specimens [27 of which (62.8%) were BRAF V600E-positive] were available. CD3(+) tumour-infiltrating lymphocytes (TILs) were evident in 33 specimens (76.7%), CD8(+) in 39 (90.7%), CD45RO(+) in 32 (74.4%), PD-1(+) in 27 (62.8%), FoxP3(+) in 21 (48.8%) and CD20(+) TILs in 19 (44.2%). Tumour PD-L1 expression was observed in 22 specimens (51.1%), and in nine of these (40.9%) expression was observed in more than 5% of tumour cells. PD-L1 expression was associated with higher density of PD-1(+) (P< 0.001), CD3(+) (P=0.014) and FoxP3(+) (P