Overexpression of polo-like kinase 1 (PLK1) and chromosomal instability in bladder cancer
Overexpression of polo-like kinase 1 (PLK1) and chromosomal instability in bladder cancer
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DOI：
10.1159/000094416
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发表时间：
2006-01-01
期刊：
影响因子：
3.5
通讯作者：
Sasaki, Kohsuke
中科院分区：
文献类型：
--
作者：
Yamamoto, Yoshiaki;Matsuyama, Hideyasu;Sasaki, Kohsuke
Polo样激酶1（PLK1）参与双极纺锤体的形成和进入有丝分裂。染色体不稳定性（CIN）是由纺锤体形成和染色体分离异常引起的。在这项研究中，我们研究了PLK1过表达与CIN的关系，并比较了PLK1状态与101例膀胱尿路上皮癌的临床病理参数。免疫组化法检测PLK1的表达和中心体的数目。通过荧光原位杂交评价了允许估计CIN的7、9和17号染色体斑点的数目畸变，并通过激光扫描细胞术评估了DNA倍体。在着丝粒拷贝数中具有大的细胞间变异的癌症被定义为CIN癌症。PLK1过表达的肿瘤与CIN（p < 0.0001）、DNA非整倍体（p = 0.0007）和中心体扩增（p = 0.0013）的相关性高于无PLK1过表达的肿瘤。PLKI的过表达与较高的病理分级（p = 0.0024）、多发肿瘤（p = 0.0241）和阳性尿细胞学（p = 0.0192）显著相关。这些数据表明PLK1的高水平表达赋予尿路上皮癌细胞肿瘤进展优势，尽管还涉及其他因素。版权所有（c）2006 S. Karger AG，巴塞尔
Polo-like kinase 1 (PLK1) participates in bipolar spindle formation and entry into mitosis. Chromosomal instability (CIN) is caused by abnormalities in spindle formation and chromosome segregation. In this study, we investigated the relationship of PLK1 overexpression to CIN, and compared the PLK1 status with clinicopathological parameters in 101 human urothelial carcinomas of the urinary bladder. Expression of PLK1 and the number of centrosomes were assessed by immunohistochemistry. Numerical aberrations of chromosomes 7, 9 and 17 spots that allowed estimation of CIN were evaluated by fluorescence in situ hybridization, and DNA ploidy was assessed by laser scanning cytometry. Cancers with a large intercellular variation in centromere copy number were defined as CIN cancers. Tumors with PLK1 overexpression were associated more frequently with CIN (p < 0.0001), DNA aneuploicly (p = 0.0007) and centrosome amplification (p = 0.0013) than those without. Overexpression of PLKI was significantly related to higher pathological grade (p = 0.0024), multiple tumors (p = 0.0241) and positive urine cytology (p = 0.0192). These data suggest that a high level expression of PLK1 confers tumor progression advantages to urothelial cancer cells, although other factors are also involved. Copyright (c) 2006 S. Karger AG, Basel