Sensitivity of cultured human osteosarcoma and chondrosarcoma cells to retinoic acid.
Sensitivity of cultured human osteosarcoma and chondrosarcoma cells to retinoic acid.
复制标题
培养的人骨肉瘤和软骨肉瘤细胞对视黄酸的敏感性。
作者：
R. Thein;R. Lotan
本文研究了维甲酸（RA）对三种人骨肉瘤细胞系（Te 85、Hs 781和Hs 791）和两种人软骨肉瘤细胞系（Hs 705和Hs 819）生长的抑制作用。暴露于10（-5）M RA导致4天内细胞形态和细胞生长的变化。RA处理的细胞出现平坦和蔓延的基质上比未处理的细胞，其指数生长率下降，其饱和密度显着降低。所有这些影响都可以通过从生长培养基中去除RA来逆转。各种细胞系表现出不同的敏感性RA的生长抑制作用。最敏感的是Hs 705软骨肉瘤。10（-9）M RA可抑制细胞增殖50%，10（-5）M RA可完全阻断细胞增殖。相反，Hs 791、Te 85、Hs 819和Hs 781的50%抑制所需的RA浓度分别为10（-7）、2 × 10（-7）、2.5 × 10（-7）和2 × 10（-6）M。只有Te 85和Hs 781骨肉瘤细胞和软骨肉瘤活检细胞能够在半固体培养基中形成集落，并且这种生长被RA显著抑制。这些结果表明，RA可以抑制在这些间充质肿瘤细胞的形态和生长特性的表达经常与转化细胞。
The ability of retinoic acid (RA) to inhibit the growth of three cell lines (Te85, Hs781, and Hs791) derived from human osteosarcomas and two cell lines (Hs705 and Hs819) derived from human chondrosarcomas was studied in culture. The exposure to 10(-5) M RA resulted, within 4 days, in changes in both cell morphology and cell growth. RA-treated cells appeared flat and spread on the substratum more than untreated cells, their exponential growth rates decreased, and their saturation densities were markedly reduced. All these effects could be reversed by removal of RA from the growth medium. The various cell lines exhibited differential susceptibility to the growth-inhibitory effect of RA. The most sensitive was the Hs705 chondrosarcoma. The proliferation of these cells was inhibited 50% by 10(-9) M RA and was completely blocked by 10(-5) m RA. In contrast, the concentrations of RA required for 50% inhibition of Hs791, Te85, Hs819, and Hs781 were 10(-7), 2 X 10(-7), 2.5 X 10(-7), and 2 X 10(-6) M, respectively. Only the Te85 and the Hs781 osteosarcoma cells and cells derived from a chondrosarcoma biopsy were able to form colonies in a semisolid medium, and this growth was dramatically inhibited by RA. These results demonstrate that RA can suppress in these mesenchymal tumor cells the expression of morphological and growth properties frequently associated with transformed cells.
13-顺式视黄酸在晚期癌症患者中的药代动力学。
影响因子：
11.2
作者：
Goodman,GE;Einspahr,JG;Alberts,DS;Davis,TP;Leigh,SA;Chen,HS;Meyskens,FL
通讯作者：
Meyskens,FL