Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor
Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor
复制标题
利用石墨烯场效应晶体管生物传感器检测全血中心力衰竭相关生物标志物
DOI：
10.1016/j.bios.2016.12.018
复制
发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Zhang, Guo-Jun
中科院分区：
文献类型：
--
作者：
Lei, Yong-Min;Xiao, Meng-Meng;Zhang, Guo-Jun
由于脑钠肽（BNP）已成为国际公认的心力衰竭（HF）诊断和预后的生物标志物，因此非常需要寻找用于在早期检测患者的BNP水平的新型传感工具。在这里，我们报告了一种铂纳米颗粒（PtNPs）修饰的还原氧化石墨烯（rGO）场效应晶体管（FET）生物传感器与微过滤系统耦合，用于无标记和高灵敏度检测全血中的BNP。通过将rGO滴铸到预制的FET芯片上并随后在石墨烯表面上组装PtNP来获得PtNP修饰的rGO FET传感器。将抗BNP结合到PtNPs表面后，成功地用抗BNP固定的FET生物传感器检测BNP。结果发现，开发的FET生物传感器能够实现100 fM的低检测限。此外，BNP被成功地检测到人全血样品处理的定制的微过滤器，表明传感器的工作在一个复杂的样品矩阵的能力。该传感器为蛋白质检测提供了一个新的传感平台，在临床样品检测中具有潜在的应用价值。
Since brain natriuretic peptide (BNP) has become internationally recognized biomarkers in the diagnosis and prognosis of heart failure (HF), it is highly desirable to search for a novel sensing tool for detecting the patient's BNP level at the early stage. Here we report a platinum nanoparticles (PtNPs)-decorated reduced graphene oxide (rGO) field effect transistor (FET) biosensor coupled with a microfilter system for label-free and highly sensitive detection of BNP in whole blood. The PtNPs-decorated rGO FET sensor was obtained by drop-casting rGO onto the pre-fabricated FET chip and subsequently assembling PtNPs on the graphene surface. After antiBNP was bound to the PtNPs surface, BNP was successfully detected by the anti-BNP immobilized FET biosensor. It was found that the developed FET biosensor was able to achieve a low detection limitation of 100 fM. Moreover, BNP was successfully detected in human whole blood sample treated by a custom-made microfilter, suggesting the sensor's capability of working in a complex sample matrix. The developed FET biosensor provides a new sensing platform for protein detection, showing its potential applications in clinic sample.