Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis.
Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis.
复制标题
Aurora在有丝分裂中依赖于中心体依赖的微管组装需要TACC3/Maskin的磷酸化。
DOI：
10.1083/jcb.200503023
复制
发表时间：
2005-09-26
影响因子：
7.8
通讯作者：
Hyman, Anthony A
中科院分区：
文献类型：
--
作者：
Kinoshita, Kazuhisa;Noetzel, Tim L;Pelletier, Laurence;Mechtler, Karl;Drechsel, David N;Schwager, Anne;Lee, Mike;Raff, Jordan W;Hyman, Anthony A
中心体是微管生长的场所，但在细胞周期中，中心体中微管的数量和稳定性是如何控制的，目前还知之甚少。我们研究了TACC 3-XMAP 215复合物在这一过程中的作用，通过使用纯化的蛋白质和非洲爪蟾卵提取物。我们表明，TACC 3形成一个一对一的复合物，并增强微管稳定活性的XMAP 215在体外。TACC 3增加了从有丝分裂中心体发出的微管的数量，并且其对中心体的靶向由Aurora A依赖性磷酸化调节。我们认为Aurora A对TACC 3活性的调节定义了一种中心体特异性机制，用于调节有丝分裂中的微管聚合。
Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3–XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.