Rat Sertoli cells acquire a beta-adrenergic response during primary culture.
Rat Sertoli cells acquire a beta-adrenergic response during primary culture.
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大鼠支持细胞在原代培养过程中获得β-肾上腺素能反应。
DOI：
10.1073/pnas.82.7.2049
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发表时间：
1985
影响因子：
11.1
通讯作者：
Perkins,JP
中科院分区：
文献类型：
--
作者：
Kierszenbaum,AL;Spruill,WA;White,MG;Tres,LL;Perkins,JP
二维聚丙烯酰胺凝胶电泳和放射性配体（-）-[125 I]碘吲哚（125 I-Pin）已被用来研究异丙肾上腺素依赖性蛋白磷酸化和β-肾上腺素能受体的可用性，分别在培养的支持细胞和新鲜分离的曲细精管段性不成熟和成熟的大鼠。从性不成熟大鼠制备的支持细胞显示与异丙肾上腺素诱导的细胞形状变化，免疫反应性波形蛋白的重新分布，和磷酸化的中间丝蛋白的原代培养物中的渐进125 I-Pin结合。放线菌酮阻断125 I-Pin与支持细胞裂解物结合的发展。生精小管不显示显著的异丙肾上腺素依赖性波形蛋白磷酸化，也不显示125 I-Pin结合。然而，波形蛋白磷酸化可由促卵泡激素或环核苷酸类似物诱导。这项研究强调，需要相关的药理学诱导的反应中观察到的支持细胞的原代培养物与那些在完整的曲细精管。
Two-dimensional polyacrylamide gel electrophoresis and the radioligand (-)-[125I]iodopindolol (125I-Pin) have been used to study isoproterenol-dependent protein phosphorylation and beta-adrenergic receptor availability, respectively, in cultured Sertoli cells and freshly isolated seminiferous tubular segments of sexually immature and mature rats. Sertoli cells prepared from sexually immature rats show progressive 125I-Pin binding in primary cultures that correlates with isoproterenol-induced cell shape changes, redistribution of immunoreactive vimentin, and phosphorylation of this intermediate filament protein. The development of 125I-Pin binding to Sertoli cell lysates is blocked by cycloheximide. Seminiferous tubules do not show significant isoproterenol-dependent vimentin phosphorylation nor 125I-Pin binding. However, vimentin phosphorylation can be induced by follicle-stimulating hormone or a cyclic nucleotide analog. This study stresses the need for correlating pharmacological-induced responses observed in Sertoli cell primary cultures with those in the intact seminiferous tubule.