Novel 6′-N-Aminoglycoside Acetyltransferase AAC(6′)-Iaj from a Clinical Isolate of Pseudomonas aeruginosa
Novel 6′-N-Aminoglycoside Acetyltransferase AAC(6′)-Iaj from a Clinical Isolate of Pseudomonas aeruginosa
复制标题
DOI：
10.1128/aac.01105-12
复制
发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Kirikae, Teruo
中科院分区：
文献类型：
--
作者：
Tada, Tatsuya;Miyoshi-Akiyama, Tohru;Kirikae, Teruo
铜绿假单胞菌NCGM 1588具有新的染色体1类整合子In 151，其包括aac（6 '）-Iaj基因。编码的蛋白质AAC（6 '）-Iaj由184个氨基酸组成，与AAC（6'）-Ia的同源性为70%。用含有aac（6 '）-Iaj基因的质粒转化的大肠杆菌获得了对除庆大霉素之外的所有测试的氨基糖苷类的抗性。值得注意的是，aac（6 '）-Iaj有助于对阿贝卡星的抗性。薄层层析显示AAC（6 '）-Iaj乙酰化除庆大霉素外的所有测试的氨基糖苷类。这些发现表明AAC（6 '）-Iaj是一种功能性乙酰转移酶，其修饰氨基糖苷类6'位的氨基，并导致铜绿假单胞菌NCGM 1588（包括阿贝卡星）的氨基糖苷类耐药性。
Pseudomonas aeruginosa NCGM1588 has a novel chromosomal class 1 integron, In151, which includes the aac(6')-Iaj gene. The encoded protein, AAC(6')-Iaj, was found to consist of 184 amino acids, with 70% identity to AAC(6')-Ia. Escherichia coli transformed with a plasmid containing the aac(6')-Iaj gene acquired resistance to all aminoglycosides tested except gentamicin. Of note, aac(6')-Iaj contributed to the resistance to arbekacin. Thin-layer chromatography revealed that AAC(6')-Iaj acetylated all aminoglycosides tested except gentamicin. These findings indicated that AAC(6')-Iaj is a functional acetyltransferase that modifies the amino groups at the 6' positions of aminoglycosides and contributes to aminoglycoside resistance of P. aeruginosa NCGM1588, including arbekacin.