Hermes, a functional non-Drosophilid insect gene vector from Musca domestica.
Hermes, a functional non-Drosophilid insect gene vector from Musca domestica.
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发表时间：
1996-03
期刊：
影响因子：
3.3
通讯作者：
D. O’brochta;W. Warren;K. Saville;P. Atkinson
中科院分区：
文献类型：
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作者：
D. O’brochta;W. Warren;K. Saville;P. Atkinson
爱马仕是来自家蝇的短反向重复型转座因子。利用染色体外转座重组试验，我们表明，爱马仕元件可以准确地转座M。南极洲的胚胎。为了测试爱马仕在与M.我们使用了含有果蝇while（w+）基因的非自主性爱马仕元件，创造了D.黑腹菌种系转化体。从显微注射w-胚发育的可育G 0成虫中回收了34.6%的转基因G1昆虫。该转化率与在D中使用P或流浪汉载体观察到的转化率相当。然而，在黑腹果蝇中，观察到许多多元素插入和大簇的情况。遗传作图、Southern杂交、多线染色体原位杂交和DNA序列分析证实了爱马仕元件在转基因昆虫染色体上整合。我们的数据表明，爱马仕元素在D.可能是该物种和具有医学和农业重要性的远缘物种中的有效基因载体和基因标记剂。
Hermes is a short inverted repeat-type transposable element from the house fly, Musca domestica. Using an extra-chromosomal transpositional recombination assay, we show that Hermes elements can accurately transpose in M. domestica embryos. To test the ability of Hermes to function in species distantly related to M. domestica we used a nonautonomous Hermes element containing the Drosophila melanogaster while (w+) gene and created D. melanogaster germline transformants. Transgenic G1 insects were recovered from 34.6% of the fertile G0 adults developing from microinjected w- embryos. This transformation rate is comparable with that observed using P or hobo vectors in D. melanogaster, however, many instances of multiple-element insertions and large clusters were observed. Genetic mapping, Southern blotting, polytene chromosome in situ hybridization and DNA sequence analyses confirmed that Hermes elements were chromosomally integrated in transgenic insects. Our data demonstrate that Hermes elements transpose at high rates in D. melanogaster and may be an effective gene vector and gene-tagging agent in this species and distantly related species of medical and agricultural importance.