TCGF Production for Cloning and Growth of Functional Human T Lymphocytes
TCGF Production for Cloning and Growth of Functional Human T Lymphocytes
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用于功能性人 T 淋巴细胞克隆和生长的 TCGF 生产
DOI：
10.1111/j.1365-3083.1980.tb00051.x
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发表时间：
1980
影响因子：
3.7
通讯作者：
Fritz H. Bach
中科院分区：
文献类型：
--
作者：
Hiroo Inouye;J. Hank;B. Alter;Fritz H. Bach
为了提高 T 细胞生长因子 (TCGF) 的效力，研究了几个变量对 TCGF 产生的影响。以下操作增强了 TCGF 的效力：首先，去除贴壁细胞并向生产培养物中添加吲哚美辛；其次，用1000拉德照射用于生产TCGF的细胞；第三，添加 Epstein-Barr 病毒转化的淋巴母细胞 (LCL) 细胞。还注意到添加经照射的饲养细胞提高了有限稀释克隆的效率。
In an effort to increase the potency of T cell growth factor (TCGF), several variables were examined for their effects on the production of TCGF. The following manipulations enhanced the potency of TCGF: first, the removal of adherent cells and addition of indomethacin to the producing cultures; second, irradiation with 1000 rads of the cells used to produce TCGF; and, third, the addition of Epstein‐Barr virus transformed lymphoblastoid (LCL) cells. It was also noted that the addition of irradiated feeder cells increased the efficiency of limiting dilution cloning.