Characterization of the transcriptional activation domains of human TEF3-1 (transcription enhancer factor 3 isoform 1)
Characterization of the transcriptional activation domains of human TEF3-1 (transcription enhancer factor 3 isoform 1)
复制标题
人类 TEF3-1（转录增强因子 3 亚型 1）转录激活域的表征。
DOI：
10.1016/j.abb.2015.02.003
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发表时间：
2015
影响因子：
3.9
通讯作者：
Xin Liu
中科院分区：
文献类型：
--
作者：
Cheng Qiao;Yajie Jiang;Cuilan Deng;Zebo Huang;Kaixuan Teng;Lan Chen;Xin Liu
TEF3-1 (transcription enhancer factor 3 isoform 1) is a human transcriptional factor, which has a N-terminal TEA/ATTS domain supposedly for DNA binding and C-terminal PRD and STY domains for transcriptional activation. Taking advantage of the efficient reporter design of yeast two-hybrid system, we.characterized the TEF3-1 domains in activating gene expression. Previously study usually mentioned that.the C-terminal domain of TEF3-1 has the transcriptional activity, however, our data shows that the peptides TEF3-11–66 and TEF3-1197–434 functioned as two independent activation domains, suggesting that Nterminal domain of TEF3-1 also has transcriptional activation capacity. Additionally, more deletions of.amino acids 197–434 showed that only the peptides TEF3-1197–265 contained the minimum sequences.for the C-terminal transcriptional activation domain. The protein structure is predicted to contain a.helix-turn-helix structure in TEF3-11–66 and four b sheets in TEF3-1197–265. Finally, after the truncated.fragments of TEF3-1 were expressed in HUVEC cells, the whole TEF3-1 and the two activation domains.could increase F-actin stress fiber, cell proliferation, migration and targeted gene expression. Further analysis and characterization of the activation domains in TEF3-1 may broaden our understanding of the.gene involved in angiogenesis and other pathological processes..
