Tubular profile of the Gallyas- and tau-positive argyrophilic threads in corticobasal degeneration: An electronmicroscopic study
Tubular profile of the Gallyas- and tau-positive argyrophilic threads in corticobasal degeneration: An electronmicroscopic study
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DOI：
10.1111/j.1440-1789.1996.tb00157.x
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发表时间：
1996-03-01
期刊：
影响因子：
2.3
通讯作者：
Arima, K
中科院分区：
文献类型：
--
作者：
Arima, K
本文观察了两例皮质基底节变性（CBD）脑组织嗜银丝（AT）和少突胶质细胞骨架改变的免疫细胞化学和超微结构特征。Gallyas阳性的少突胶质细胞核周卷曲体与抗tan抗体和泛素抗体呈阳性反应，超微结构由15 ~ 20个微管组成，偶见不规则的收缩。本研究首次证实：（1）非生理性的管状结构实际上聚集在髓鞘的内外环，与组织学AT相对应;（ii）这些小管的直径为15至20 nm，在超微结构上偶尔有不规则的收缩，在Gallyas电子显微镜下涂覆有银颗粒，抗tau阳性，抗遍在蛋白抗体免疫细胞化学呈弱阳性;和（iii）围绕这些异常AT小管的内外环的质膜被抗tan和抗遍在蛋白抗体免疫标记，嗜银丝和少突胶质细胞核周编码体具有相同的免疫组织化学、免疫细胞化学和电子显微镜特征。因此，少突胶质细胞从胞体开始，在其整个过程中可能发生了共同的tau蛋白病变。总之，少突胶质细胞过程中的细胞骨架病变在CBD的病因学中具有重要意义。
Two brains with corticobasal degeneration (CBD) were examined in order to elucidate the immunocytochemical and ultrastructural characteristics of argyrophilic threads (AT) and oligodendroglial cytoskeletal alterations, Gallyas-positive oligodendroglial perikaryal coiled bodies reacted positively with antibodies against tan and ubiquitin immunohistochemically, and consisted of 15 to 20 mn tubules with occasional irregular constrictions ultrastructurally. The present study demonstrated, for the first time, that (i) the tubular structure of the non-physiological nature actually aggregated in the inner and outer loops of the myelin sheath, which corresponded to the histological AT; (ii) these tubules were 15 to 20 nm in diameter with occasional irregular constrictions ultrastructurally, coated with silver particles in Gallyas electronmicroscopy, positive for anti-tau, and weakly positive for anti-ubiquitin antibodies immunocytochemically; and (iii) the plasma membrane of the inner and outer loops that surrounded these abnormal tubules of AT was immunolabeled by both anti-tan and anti-ubiquitin antibodies, Argyrophilic threads and oligodendroglial perikaryal coded bodies share the immunohistochemical, immunocytochemical, and electronmicroscopical characteristics. Therefore, it is considered that the common tau-pathology may develop in the oligodendroglia from the perikarya throughout its process, In conclusion, the cytoskeletal pathology in oligodendroglial process is of significance in the pathoetiology of CBD.