Differential effects of restraint stress on hippocampal 5-HT metabolism and extracellular levels of 5-HT in streptozotocin-diabetic rats
Differential effects of restraint stress on hippocampal 5-HT metabolism and extracellular levels of 5-HT in streptozotocin-diabetic rats
复制标题
束缚应激对链脲佐菌素糖尿病大鼠海马5-HT代谢和细胞外5-HT水平的差异影响
DOI：
10.1016/s0006-8993(97)00841-x
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发表时间：
1997
期刊：
影响因子：
2.9
通讯作者：
Y. Michotte
中科院分区：
文献类型：
--
作者：
K. Thorré;F. Chaouloff;S. Sarre;R. Meeusen;G. Ebinger;Y. Michotte
链脲佐菌素(STZ)诱导的糖尿病降低了中枢5-羟色胺(5-羟色胺，5-HT)的合成/代谢，但这种减少是否会导致5-HT的释放减少还鲜有研究。因此，我们分析了STZ糖尿病在基础条件下和束缚应激期间对海马细胞外5-羟色胺水平的影响，束缚应激是一种刺激海马5-羟色胺合成/代谢和释放的程序。5-羟色胺和5-羟基吲哚乙酸的组织化学分析显示，预先给予STZ(提前3周)和束缚1h分别降低和增加了海马5-羟色胺的代谢。另一方面，海马区微透析显示，在(清醒的)赋形剂和STZ预处理的大鼠中，细胞外5-羟色胺水平的基础水平没有差异，但有不同的束缚效应。因此，在整个束缚过程中，赋形剂组的细胞外5-羟色胺水平增加(最大增加：194%)，但在STZ预处理的大鼠中没有增加。然而，在后一组大鼠中，血浆皮质酮水平升高，因此表明对压力有明显的厌恶。最后，因为焦虑相关的行为可能会受到海马五羟色胺能系统的影响，所以在焦虑的高架+迷宫中，比较了静息和受限的载体和STZ预处理的大鼠(紧接着应激后)。STZ预处理减少了张开手臂和闭合手臂的百分比，分别表明焦虑增加和运动活动减少。克制倾向于增加所有大鼠的焦虑相关行为，但这一趋势从未达到显著水平。我们的结果证实，对5-羟色胺代谢的大体分析并不能提供有关5-羟色胺释放的信息，并表明情感障碍患者中糖尿病的流行可能与缺乏对厌恶刺激的海马5-羟色胺反应有关。
Streptozotocin (STZ)-elicited diabetes reduces central serotonin (5-hydroxytryptamine, 5-HT) synthesis/metabolism, but whether this reduction leads to decreased release of 5-HT has only scarcely been investigated. We have thus analysed the impact of STZ diabetes on hippocampal extracellular 5-HT levels both under basal conditions and during restraint stress, a procedure known to stimulate hippocampal 5-HT synthesis/metabolism and release. The pretreatment with STZ (3 weeks beforehand) and the 1 h restraint session respectively decreased and increased hippocampal 5-HT metabolism, as assessed by tissue analysis of 5-HT and 5-hydroxyindoleacetic acid. On the other hand, hippocampal microdialysis revealed no difference in basal levels of extracellular 5-HT levels in (conscious) vehicle- and STZ-pretreated rats, but a differential effect of restraint. Thus, extracellular 5-HT levels increased throughout restraint (maximal increase: 194%) in vehicle-, but not in STZ-pretreated rats. In the latter rat group, plasma corticosterone levels were, however, increased, thus indicating a significant aversiveness to stress. Lastly, because anxiety-related behaviours may be affected by hippocampal serotonergic systems, resting and restrained vehicle- and STZ-pretreated rats were compared (immediately after stress) in an elevated plus-maze of anxiety. Pretreatment with STZ reduced the percent number of open arm entries and the number of closed arm entries, indicating increased anxiety and reduced locomotor activity, respectively. Restraint tended to increase anxiety-related behaviours in all rats, but this trend never reached significance. Our results confirm that gross analyses of 5-HT metabolism do not yield information on 5-HT release, and suggest that the prevalence of diabetes among patients suffering affective disorders could be related to the lack of hippocampal serotonergic response to aversive stimuli.
大鼠慢性链脲佐菌素糖尿病可促进急性应激反应，而不改变垂体或肾上腺对促分泌剂的反应性。
影响因子：
4.8
作者：
Scribner,KA;Walker,CD;Cascio,CS;Dallman,MF
通讯作者：
Dallman,MF
应激性住房条件与小鼠多次亚糖尿病剂量链脲佐菌素诱导的糖尿病发病的关系。
DOI：
--
发表时间：
1987
期刊：
Diabetes research (Edinburgh, Scotland)
影响因子：
--
作者：
Mazelis,AG;Albert,D;Crisa,C;Fiore,H;Parasaram,D;Franklin,B;Ginsberg-Fellner,F;McEvoy,RC
通讯作者：
McEvoy,RC